Mucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinoma

Simple item page
datacite.alternateIdentifier.citationHISTOPATHOLOGY, Vol. 74, Nº 4, 597-607, 2019en_US
datacite.alternateIdentifier.doi10.1111/his.13797en_US
datacite.alternateIdentifier.issn1365-2559 (electrónica)en_US
datacite.creatorEspinoza, Jaime A.
datacite.creatorRiquelme, Ismael
datacite.creatorSagredo, Eduardo A.
datacite.creatorRosa, Lorena
datacite.creatorGarcía, Patricia
datacite.creatorBizama, Carolina
datacite.creatorApud-Bell, María
datacite.creatorleal, Pamela
datacite.creatorWeber, Helga
datacite.creatorBenavente, Felipe
datacite.creatorVargas, Sergio
datacite.creatorRomero, Diego
datacite.creatorKalergis, Alexis M.
datacite.creatorRoa, Juan Carlos
datacite.date2019-03
datacite.subjectTheranostic
datacite.subjectMUC5B
datacite.subjectGallbladder cancer
datacite.subjectCA9
datacite.subjectCLDN18
datacite.titleMucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinomaen_US
dc.coverageChileen_US
dc.date.accessioned2024-03-11T15:28:20Z
dc.date.available2024-03-11T15:28:20Z
dc.description.abstractAims Gallbladder cancer (GBC) is an aggressive tumour that is usually diagnosed at advanced stages and is characterised by a poor prognosis. Using public data of normal human tissues, we found that mRNA and protein levels of mucin 5B (MUC5B) and carbonic anhydrase 9 (CA9) were highly increased in gallbladder tissues. In addition, previous evidence has shown that claudin 18 (CLDN18) protein expression is higher in GBC. The aim of this study was to perform an analysis of these cell surface proteins during the histological progression of GBC in order to identify their theranostic potential. Methods and results MUC5B expression, CA9 expression and CLDN18 expression were examined by immunohistochemistry in a series of 179 chronic cholecystitis (including 16 metaplastic tissues), 15 dysplasia and 217 GBC samples by the use of tissue microarray analysis. A composite staining score was calculated from staining intensity and percentage of positive cells. Immunohistochemical analysis showed high expression of MUC5B and CA9 among normal epithelium, metaplastic tissues, and dysplastic tissues. However, expression of both proteins was observed in roughly 50% of GBC samples. In contrast, CLDN18 was absent in normal epithelium, but its expression was higher in metaplastic cells. Among GBC cases, approximately half showed high CLDN18 expression. No associations were found between MUC5B, CA9 and CLDN18 expression and any clinicopathological features. Conclusions CLDN18 is a new metaplasia marker in gallbladder tissues, and is conserved in approximately half of GBC cases. MUC5B and CA9 are highly conserved during GBC histological progression. The three markers are potential theranostic markers, in particular CA9 and CLDN18, for which there are already targeted therapies available.en_US
dc.formatPDFen_US
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5514
dc.language.isoenen_US
dc.publisherWiley-Blackwell Publishingen_US
dc.rightsTodos los derechos reservadosen_US
dc.sourceHISTOPATHOLOGYen_US
oaire.resourceTypeArtículo de Revistaen_US
oaire.versionpostprinten_US
uct.carreraTecnología Médicaen_US
uct.catalogadorJCCLen_US
uct.comunidadCiencias de la Saluden_US
uct.disciplinaTecnología Médicaen_US
uct.facultadFacultad de Ciencias de la Saluden_US
uct.indizacionSCI EXPANDEDen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
R_Dig_UCT_R_Dig_UCT_Mucin 5B, carbonic anhydrase 9.pdf
Size:
134.84 KB
Format:
Adobe Portable Document Format
Description:
Artículo área de Tecnología Médica
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
803 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Procesos Diagnósticos y Evaluación