In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus

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dc.contributor.authorVelásquez Briceno, Alejandro
dc.contributor.authorPichard, Gaston
dc.date2010
dc.date.accessioned2021-04-30T16:35:21Z
dc.date.available2021-04-30T16:35:21Z
dc.description.abstractA. Velasquez, and G. Pichard. 2010. In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus. Cien. Inv. Agr. 37(3): 57-70. Proteolytic activity of enzymatic extracts generated from rumen microorganisms cultivated in vitro was evaluated. The incubation of rumen fluid used different substrates to generate a higher enzyme concentration and promote a broad spectrum of hydrolytic activity. The composition of the substrates used in the cultivation of the fluid was enriched in protein, starch or cell wall. Enzyme preparations were evaluated by incubating in 30 mL of buffer 50 mM Tris-HCl (pH 6.5) at 39 degrees C during 48 hours, 100 mg of crude protein from feeds soybean meal, canola meal, sunflower meal, gluten feed, dehydrated alfalfa meal, berseem clover, oat forage and perennial ryegrass. Enzyme extracts from cultivated rumen fluid showed an average protein breakdown of 75.5%, in eight feed samples tested. This value was very close to that measured with the technique of proteases from Streptomyces griseus (74.6% CP), but significantly lower (P <= 0.05) than the one obtained by the in situ methodology (84.8% CP). The technique with extracted rumen enzymes showed higher level of proteolysis in the early hours of incubation (6 H) compared to the other techniques. These results suggest that the enzyme preparations of ruminal origin have the ability to predict degradability of feed proteins in the rumen, particularly in the first phase when most of proteins are hydrolyzed and become available for microbial utilization.
dc.identifier.citationCIENCIA E INVESTIGACION AGRARIA,Vol.37,57-70,2010
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3130
dc.language.isoen
dc.publisherPONTIFICIA UNIV CATOLICA CHILE. FAC AGRONOMIA INGENIERIA FORESTAL
dc.sourceCIENCIA E INVESTIGACION AGRARIA
dc.subject.englishIn vitro rumen proteolysis
dc.subject.englishruminal enzymes
dc.subject.englishStreptomyces griseus
dc.subject.englishin situ method
dc.titleIn vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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