Differential mechanisms between traditionally established and new highbush blueberry (Vaccinium corymbosum L.) cultivars reveal new insights into manganese toxicity resistance
Date
Authors
GONZALEZ VILLAGRA, JORGE ANDRES
INOSTROZA BLANCHETEAU, CLAUDIO ANDRES
González-Villagra, Jorge
Escobar, Ana Luengo
Ribera, Alejandra E.
Cárcamo, María Paz
Omena-Garcia, Rebeca Patrícia
Nunes-Nesi, Adriano
Inostroza-Blancheteau, Claudio
Alberdi, Miren R.
Reyes-Díaz, Marjorie M.
INOSTROZA BLANCHETEAU, CLAUDIO ANDRES
González-Villagra, Jorge
Escobar, Ana Luengo
Ribera, Alejandra E.
Cárcamo, María Paz
Omena-Garcia, Rebeca Patrícia
Nunes-Nesi, Adriano
Inostroza-Blancheteau, Claudio
Alberdi, Miren R.
Reyes-Díaz, Marjorie M.
Authors
Date
Datos de publicación:
10.1016/j.plaphy.2020.11.034
Keywords
Co2 Assimilation - Mn Transporter - Oxidative Stress - Plant Growth - Stomatal Density - Manganese - Manganese - Manganese - Blueberry - Chile - Classification - Drug Effect - Gene Expression Regulation - Physiology - Plant Leaf - Blueberry Plants - Gene Expression Regulation, Plant - Manganese - Plant Leaves
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Abstract
In acid soils, manganese (Mn) concentration increases, becoming toxic to plants. Mn toxicity differentially affects physiological processes in highbush blueberry (Vaccinium corymbosum L.) cultivars. However, the mechanisms involved in Mn toxicity of the new and traditionally established cultivars are unknown. To understand Mn toxicity mechanisms, four traditionally established (Legacy, Brigitta, Duke, and Star) cultivars and two recently introduced to Chile (Camellia and Cargo) were grown under hydroponic conditions subjected to control Mn (2 ?M) and Mn toxicity (1000 ?M). Physiological, biochemical, and molecular parameters were evaluated at 0, 7, 14, and 21 days. We found that the relative growth rate was reduced in almost all blueberry cultivars under Mn toxicity, except Camellia, with Star being the most affected. The photosynthetic parameters were reduced only in Star by Mn treatment. Leaf Mn concentrations increased in all cultivars, exhibiting the lowest levels in Camellia and Cargo. Brigitta and Duke exhibited higher ?-carotene levels, while Cargo exhibited a reduction under toxic Mn. In Legacy, lutein levels increased under Mn toxicity. Traditionally established cultivars exhibited higher antioxidant activity than the new cultivars under Mn toxicity. The Legacy and Duke cultivars increased VcMTP4 expression with Mn exposure time. A multivariate analysis separated Legacy and Duke from Camellia; Star and Cargo; and Brigitta. Our study demonstrated that Mn toxicity differentially affects physiological, biochemical, and molecular features in the new and traditionally established cultivars, with Legacy, Duke, Camellia, and Cargo as the Mn-resistant cultivars differing in their Mn-resistance mechanisms and Star as the Mn-sensitive cultivar. © 2020 Elsevier B.V., All rights reserved.
Description
Keywords
Co2 Assimilation , Mn Transporter , Oxidative Stress , Plant Growth , Stomatal Density , Manganese , Manganese , Manganese , Blueberry , Chile , Classification , Drug Effect , Gene Expression Regulation , Physiology , Plant Leaf , Blueberry Plants , Gene Expression Regulation, Plant , Manganese , Plant Leaves
Citation
10.1016/j.plaphy.2020.11.034