Marker-assisted breeding for TaALMT1, a major gene conferring aluminium tolerance to wheat

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dc.contributor.authorSoto Cerda, B. J.
dc.contributor.authorInostroza Blancheteau, C.
dc.contributor.authorMathias, M.
dc.contributor.authorPenaloza, E.
dc.contributor.authorZuniga, J.
dc.contributor.authorMunoz, G.
dc.contributor.authorRengel, Z.
dc.contributor.authorSalvo Garrido, H.
dc.date2015
dc.date.accessioned2021-04-30T17:06:05Z
dc.date.available2021-04-30T17:06:05Z
dc.description.abstractAluminium toxicity in acid soils is the main limitation to crop production worldwide. In wheat (Triticum aestivum L.), the Al-activated malate transporter (TaALMT1) gene located on chromosome 4DL is associated with malate efflux and Al-tolerance. To introgress Al-tolerance from the breeding line CAR3911 into the high yielding Al-sensitive cultivar Kumpa-INIA, phenotypic and molecular characterizations of gene/QTL underlying Al-tolerance in CAR3911 followed by marker-assisted backcrossing (MAS-BC) were undertaken. Al-tolerant backcross (BC) lines were selected using the functional marker ALMT1-4 designed immediately upstream of the TaALMT1 coding region. Foreground and background selections using ALMT1-4 and microsatellite markers were conducted. Linkage and sequence analyses suggest that the TaALMT1 gene could underly the Al-tolerance in CAR3911, possessing the same promoter type (V) as the Al-tolerant genotypes Carazinho and ET8. The MAS-BC strategy allowed the selection of Al-tolerant lines with the smallest introgressed region (6 cM) on 4D and the highest recurrent parent genome (RPG) (98 %) covering 2 194 cM of the wheat genome. The homozygous BC3F2 line named Kumpa-INIA-TaALMT1 expressed a 3-fold higher Al-tolerance than its isogenic line Kumpa-INIA at 40 mu M Al in the hydroponic solution, and similarly to CAR3911 and Carazinho. The MAS-BC strategy was successful for the introgression of the TaALMT1 gene into Kumpa-INIA in only three BC generations, shortening the breeding cycle to 24 months, which promises to increase wheat production and a greater yield stability in the acid soils of Southern Chile.
dc.identifier.citationBIOLOGIA PLANTARUM,Vol.59,83-91,2015
dc.identifier.doi10.1007/s10535-014-0474-x
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/4001
dc.language.isoen
dc.publisherACAD SCIENCES CZECH REPUBLIC. INST EXPERIMENTAL BOTANY
dc.sourceBIOLOGIA PLANTARUM
dc.subject.englishacid soils
dc.subject.englishbackcross lines
dc.subject.englishgene expression
dc.subject.englishintrogression
dc.subject.englishmalate transporter
dc.subject.englishmicrosatellite markers
dc.titleMarker-assisted breeding for TaALMT1, a major gene conferring aluminium tolerance to wheat
dc.typeArticle
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uct.indizacionSCI
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