Characterization of Toxoplasma gondii isolates in free-range chickens from Chile, South America

dc.contributor.authorDubey, J. P.
dc.contributor.authorPatitucci, A.N.
dc.contributor.authorSu, C.
dc.contributor.authorSundar, N.
dc.contributor.authorKwok, O. C. H.
dc.contributor.authorShen, S. K.
dc.date2006
dc.date.accessioned2012-02-29T21:21:12Z
dc.date.available2012-02-29T21:21:12Z
dc.date.issued2012-02-29
dc.description.abstractThe prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 85 free-range chickens (Gallus domesticus) from Chile was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT), and found in 47 of 85 (55.3.9%) chickens with titers of 1:5 in six, 1:10 in four, 1:20 in four 1: 40 in three, 1: 80 in nine, 1: 160 in four 1:320 in nine, and 1: 640 or higher in eight. Hearts and brains of 47 chickens with titers of 1:5 or higher were pooled for each chicken and bioassayed in mice. Tissues from 16 seronegative (MAT < 1:5) chickens were pooled and fed to one T. gondii-free cat. Feces of the cat were examined for oocysts but none was found based on bioassay of fecal floats in mice. Hearts and brains from seven seronegative (<1:5) were pooled and bioassayed in mice; T. gondii was not isolated. T. gondii was isolated by bioassay in mice from 22 chickens with MAT titers of 1:20 or higher. Genotyping of these 22 isolates using polymorphisms at the loci SAG1, SAG2, SAG3, BTUB and GRA6 revealed three genotypes. Seventeen isolates had type II alleles and four isolates had type III alleles at all loci. One isolate contained the combination of type I and III alleles. This is the first report of genetic characterization of T. gondii isolates from Chile, South America.es
dc.formatPDFes
dc.identifier.citationVeterinary Parasitology, Vol.140, N1-2, 76-82, 2006es
dc.identifier.doi10.1016/j.vetpar.2006.03.023es
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/808
dc.language.isoenes
dc.sourceVeterinary Parasitologyes
dc.subjectAveses
dc.subjectToxoplasma gondiies
dc.titleCharacterization of Toxoplasma gondii isolates in free-range chickens from Chile, South Americaes
dc.typeArtículo de Revistaes
uct.carreraMedicina Veterinariaes
uct.catalogadorFGEes
uct.comunidadRecursos Naturaleses
uct.facultadFacultad de Recursos Naturaleses
uct.indizacionISIes
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