Dubey, J. P.; Patitucci, A.N.; Su, C.; Sundar, N.; Kwok, O. C. H.; Shen, S. K.

Characterization of Toxoplasma gondii isolates in free-range chickens from Chile, South America

datacite.alternateIdentifier.citation	Veterinary Parasitology, Vol.140, N1-2, 76-82, 2006	es
datacite.alternateIdentifier.doi	10.1016/j.vetpar.2006.03.023	es
datacite.creator	Dubey, J. P.
datacite.creator	Patitucci, A.N.
datacite.creator	Su, C.
datacite.creator	Sundar, N.
datacite.creator	Kwok, O. C. H.
datacite.creator	Shen, S. K.
datacite.date	2006
datacite.date.issued	2012-02-29
datacite.subject	Aves	es
datacite.subject	Toxoplasma gondii	es
datacite.title	Characterization of Toxoplasma gondii isolates in free-range chickens from Chile, South America	es
dc.date.accessioned	2012-02-29T21:21:12Z
dc.date.available	2012-02-29T21:21:12Z
dc.description.abstract	The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 85 free-range chickens (Gallus domesticus) from Chile was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT), and found in 47 of 85 (55.3.9%) chickens with titers of 1:5 in six, 1:10 in four, 1:20 in four 1: 40 in three, 1: 80 in nine, 1: 160 in four 1:320 in nine, and 1: 640 or higher in eight. Hearts and brains of 47 chickens with titers of 1:5 or higher were pooled for each chicken and bioassayed in mice. Tissues from 16 seronegative (MAT < 1:5) chickens were pooled and fed to one T. gondii-free cat. Feces of the cat were examined for oocysts but none was found based on bioassay of fecal floats in mice. Hearts and brains from seven seronegative (<1:5) were pooled and bioassayed in mice; T. gondii was not isolated. T. gondii was isolated by bioassay in mice from 22 chickens with MAT titers of 1:20 or higher. Genotyping of these 22 isolates using polymorphisms at the loci SAG1, SAG2, SAG3, BTUB and GRA6 revealed three genotypes. Seventeen isolates had type II alleles and four isolates had type III alleles at all loci. One isolate contained the combination of type I and III alleles. This is the first report of genetic characterization of T. gondii isolates from Chile, South America.	es
dc.format	PDF	es
dc.identifier.uri	https://repositoriodigital.uct.cl/handle/10925/808
dc.language.iso	en	es
dc.source	Veterinary Parasitology	es
oaire.resourceType	Artículo de Revista	es
uct.carrera	Medicina Veterinaria	es
uct.catalogador	FGE	es
uct.comunidad	Recursos Naturales	es
uct.facultad	Facultad de Recursos Naturales	es
uct.indizacion	ISI	es