Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)

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datacite.alternateIdentifier.citationZYGOTE,Vol.20,327-331,2012
datacite.alternateIdentifier.doi10.1017/S0967199411000682
datacite.creatorValdebenito Isler, Iván
datacite.creatorSanchez, Ruben R.
datacite.creatorEffer Roldan, Brian
datacite.creatorUbilla Madrid, Andrea
datacite.date2012
datacite.subject.englishAsymmetrical
datacite.subject.englishEarly stage
datacite.subject.englishFirst blastomeres
datacite.subject.englishRainbow trout
datacite.subject.englishSymmetrical
datacite.titleMorphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)
dc.date.accessioned2021-04-30T16:34:19Z
dc.date.available2021-04-30T16:34:19Z
dc.description.abstractIn the following investigation the morphometric characteristics of the first two blastomeres of rainbow trout (Oncorhynchus mykiss) were determined. Embryos were incubated at 9 degrees C and then fixed in a Stockard solution every 30 min starting from 8.5 to 12.5 h of incubation post fertilization. Embryonic discs were extracted and microphotographs were taken with Q Capture Pro 5.0 software using a stereomicroscope Olympus SZX7. The average size of the blastodiscs was 941.22 +/- 160.42 mu m. The first cleavage finished after approximately 12 h of incubation. The first two blastomeres were regularly symmetrical in their morphology. Blastomere 1 had an average length (L) of 942.68 +/- 105.56 mu m and width (W) of 467.34 +/- 64.33 mu m. Blastomere 2 had an average length of 887.60 +/- 101.65 and width of 454.49 +/- 47.25 mu m (n = 91). Significant differences were found between the length and width of blastomeres 1 and 2. The proportion between the length of blastomeres 1 and 2 was 0.94 +/- 0.07 (n = 91); between the width of blastomeres 1 and 2 it was 0.88 +/- 0.11 (n = 91); and the width/length ratio was 0.51 +/- 0.09 (n = 182). It was concluded that rainbow trout blastomeres tend to be asymmetrical in length with a higher dispersion of widths.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3031
dc.language.isoen
dc.publisherCAMBRIDGE UNIV PRESS
dc.sourceZYGOTE
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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