Aspergillus fumigatus-sensitive IgE is associated with bronchial hypersensitivity in a murine model of neutrophilic airway inflammation

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datacite.alternateIdentifier.citationJournal de Mycologie Medicale, Vol.28, N°1, 128-136, 2018en_US
datacite.alternateIdentifier.doi10.1016/j.mycmed.2017.11.005en_US
datacite.creatorMorán, G
datacite.creatorOrtloff Trautmann, Alexander
datacite.creatorMorán, G.
datacite.creatorFolch, H.
datacite.creatorUberti, B.
datacite.date2018
datacite.subjectAspergillus fumigatusen_US
datacite.subjectAsma neutrofílicaen_US
datacite.titleAspergillus fumigatus-sensitive IgE is associated with bronchial hypersensitivity in a murine model of neutrophilic airway inflammationen_US
dc.date.accessioned2020-04-06T21:40:36Z
dc.date.available2020-04-06T21:40:36Z
dc.description.abstractNeutrophils are the predominant inflammatory cells that infiltrate airways during acute exacerbation of asthma. The importance of A. fumigatus sensitization, and IgE response in the airways in patients with acute asthma is unclear. Rockefeller (RK) mice were sensitized with A. fumigatus extract protein. The animals were subsequently challenged with different degrees of A. fumigatus contamination in the cage bedding. All groups of mice were euthanized to obtain bronchoalveolar lavage fluid (BALF) for cytological and Elisa assays, and lung tissue for histological analysis. Moreover, several bioassays were conducted to determine whether BALF IgE antibodies can activate mast cells. In this study, we demonstrated that exposure of sensitized mice to a known concentration of A. fumigatus conidia produces bronchial hyperreactivity with marked neutrophilic bronchial infiltration and increased BALF IgE, capable of triggering mast cell degranulation. This study suggests that IgE may play a role in bronchial hyperreactivity associated to A. fumigatus exposure in mice. Mice sensitized and challenged with this fungus showed characteristics of severe asthma, with an increase of BALF neutrophils, histological changes consistent with severe asthma and an increase of IgE capable of triggering type I hypersensitivityen_US
dc.formatPDFen_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2150
dc.language.isoenen_US
dc.sourceJournal de Mycologie Medicaleen_US
oaire.resourceTypeArtículo de Revistaen_US
uct.catalogadorpopen_US
uct.comunidadCiencias de la Saluden_US
uct.indizacionISI - Science Citation Indexen_US
uct.indizacionSCOPUSen_US
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