Intestinal transcriptome analysis reveals enrichment of genes associated with immune and lipid mechanisms, favoring soybean meal tolerance in high-growth zebrafish (Danio rerio)
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Authors
HERNANDEZ ARIAS, ADRIAN JESUS
DANTAGNAN DANTAGNAN, HERMAN PATRICIO
Valenzuela, Luis
Pacheco, Sebastian
Rincón, Gonzalo
Pavez, Leonardo
Lam, Natalia
Hernández Arias, Adrián J.
Dantagnan, Patricio P.
González, Felipe
Jilberto, Felipe
Cristina Ravanal, María
DANTAGNAN DANTAGNAN, HERMAN PATRICIO
Valenzuela, Luis
Pacheco, Sebastian
Rincón, Gonzalo
Pavez, Leonardo
Lam, Natalia
Hernández Arias, Adrián J.
Dantagnan, Patricio P.
González, Felipe
Jilberto, Felipe
Cristina Ravanal, María
Authors
Date
Datos de publicación:
10.3390/genes12050700
Keywords
High-growth Fish - Rna-seq - Soybean Meal Tolerance - Sustainable Aquaculture - Transcriptome - Zebrafish - Cholesterol 24 Hydroxylase - Cholesterol 7alpha Monooxygenase - Soybean Protein - Soybean Proteins - Zebrafish Proteins - Cholesterol 24 Hydroxylase - Cholesterol 7alpha Monooxygenase - Retinol Binding Protein 4 - Soybean Protein - Transcriptome - Zebrafish Protein - Analysis Of Variance - Article - Body Weight Gain - Cftr Gene - Controlled Study - Cxcr3 2 Gene - Cyp46a1 Gene - Down Regulation - Elov5l Gene - Elov6l Gene - Gene - Gene Expression Profiling - Gene Ontology - Genetic Association - Genetic Variation - Genotype Environment Interaction - Il22 Gene - Insulin Response - Irg1l Gene - Itln2 Gene - Lipid Metabolism - Mbtps1 Gene - Mpx Gene - Nonhuman - Nr2f5 Gene - Nup61l Gene - Principal Component Analysis - Rbp4 Gene - Rna Sequence - Scoring System - Sgk1 Gene - Signal Transduction - Soybean Meal - Stra6 Gene - Transcriptomics - Upregulation - Zebra Fish - Animal - Genetics - Immunology - Innate Immunity - Intestine Mucosa - Metabolism - Animals - Immunity, Innate - Intestinal Mucosa - Lipid Metabolism - Signal Transduction - Soybean Proteins - Transcriptome - Zebrafish - Zebrafish Proteins
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Abstract
The molecular mechanisms underlying fish tolerance to soybean meal (SBM) remain unclear. Identifying these mechanisms would be beneficial, as this trait favors growth. Two fish replicates from 19 experimental families were fed fishmeal-(100FM) or SBM-based diets supplemented with saponin (50SBM + 2SPN) from juvenile to adult stages. Individuals were selected from families with a genotype-by-environment interaction higher (HG-50SBM + 2SPN, 170 ± 18 mg) or lower (LG-50SBM + 2SPN, 76 ± 10 mg) weight gain on 50SBM + 2SPN for intestinal transcriptomic analysis. A histological evaluation confirmed middle intestinal inflammation in the LG-vs. HG-50SBM + 2SPN group. Enrichment analysis of 665 differentially expressed genes (DEGs) identified pathways associated with immunity and lipid metabolism. Genes linked to intestinal immunity were downregulated in HG fish (mpx, cxcr3.2, cftr, irg1l, itln2, sgk1, nup61l, il22), likely dampening inflammatory responses. Conversely, genes involved in retinol signaling were upregulated (rbp4, stra6, nr2f5), potentially favoring growth by suppressing insulin responses. Genes associated with lipid metabolism were upregulated, including key components of the SREBP (mbtps1, elov5l, elov6l) and cholesterol catabolism (cyp46a1), as well as the downregulation of cyp7a1. These results strongly suggest that transcriptomic changes in lipid metabolism mediate SBM tolerance. Genotypic variations in DEGs may become biomarkers for improving early selection of fish tolerant to SMB or others plant-based diets. © 2021 Elsevier B.V., All rights reserved.
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Keywords
High-growth Fish , Rna-seq , Soybean Meal Tolerance , Sustainable Aquaculture , Transcriptome , Zebrafish , Cholesterol 24 Hydroxylase , Cholesterol 7alpha Monooxygenase , Soybean Protein , Soybean Proteins , Zebrafish Proteins , Cholesterol 24 Hydroxylase , Cholesterol 7alpha Monooxygenase , Retinol Binding Protein 4 , Soybean Protein , Transcriptome , Zebrafish Protein , Analysis Of Variance , Article , Body Weight Gain , Cftr Gene , Controlled Study , Cxcr3 2 Gene , Cyp46a1 Gene , Down Regulation , Elov5l Gene , Elov6l Gene , Gene , Gene Expression Profiling , Gene Ontology , Genetic Association , Genetic Variation , Genotype Environment Interaction , Il22 Gene , Insulin Response , Irg1l Gene , Itln2 Gene , Lipid Metabolism , Mbtps1 Gene , Mpx Gene , Nonhuman , Nr2f5 Gene , Nup61l Gene , Principal Component Analysis , Rbp4 Gene , Rna Sequence , Scoring System , Sgk1 Gene , Signal Transduction , Soybean Meal , Stra6 Gene , Transcriptomics , Upregulation , Zebra Fish , Animal , Genetics , Immunology , Innate Immunity , Intestine Mucosa , Metabolism , Animals , Immunity, Innate , Intestinal Mucosa , Lipid Metabolism , Signal Transduction , Soybean Proteins , Transcriptome , Zebrafish , Zebrafish Proteins
Citation
10.3390/genes12050700