Genotoxicity testing of two lead-compounds in somatic cells of Drosophila melanogaster

dc.date2011
dc.date.accessioned2012-02-05T19:54:11Z
dc.date.available2012-02-05T19:54:11Z
dc.date.issued2012-02-05
dc.description.abstractThe in vivo genotoxic activity of two inorganic lead compounds was studied in Drosophila melanogaster by measurement of two different genetic endpoints. We used the wing-spot test and the comet assay. The comet assay was conducted with larval haemocytes. The results from the wing-spot test showed that neither lead chloride, PbCl2, nor lead nitrate, Pb(NO3)2, were able to induce significant increases in the frequency of mutant spots. In addition, the combined treatments with gamma-radiation and PbCl2 or Pb(NO3)2 did not show significant variations in the frequency of the three categories of mutant spots recorded, compared with the frequency induced by gamma-radiation alone. This seems to indicate that the lead compounds tested do not interact with the repair of the genetic damage induced by ionizing radiation. When the lead compounds were evaluated in the in vivo comet assay with haemocytes, Pb(NO3)2 was effective in inducing significant increases of DNA damage with a direct dose-response pattern. These results confirm the usefulness of the comet assay with haemocytes as an in vivo model and support the assumption that there is a genotoxic risk associated with lead exposure. © 2011 Elsevier B.Ves
dc.formatPDFes
dc.identifier.citationMutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 724, 01-feb, 35-40, 2011es
dc.identifier.doi10.1016/j.mrgentox.2011.05.008es
dc.identifier.urihttps://hdl.handle.net/10925/552
dc.language.isoenes
dc.sourceMutation Research - Genetic Toxicology and Environmental Mutagenesises
dc.subjectGenotoxicidades
dc.subjectMutaciónes
dc.subjectDrosophila melanogasteres
dc.titleGenotoxicity testing of two lead-compounds in somatic cells of Drosophila melanogasteres
dc.typeArtículo de Revistaes
uct.carreraAgronomíaes
uct.carreraIngeniería en Recursos Naturales Renovableses
uct.catalogadoragaes
uct.comunidadRecursos Naturaleses
uct.facultadFacultad de Recursos Naturaleses
uct.indizacionISIes
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