Novel Apoplastic Antifreeze Proteins of Deschampsia antarctica as Enhancer of Common Cell Freezing Media for Cryobanking of Genetic Resources, a Preliminary Study

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datacite.alternateIdentifier.citationBIOMOLECULES,Vol.14,2024
datacite.alternateIdentifier.doi10.3390/biom14020174
datacite.creatorShort, Stefania E.
datacite.creatorZamorano, Mauricio
datacite.creatorAranzaez Rios, Cristian
datacite.creatorLee Estevez, Manuel
datacite.creatorDiaz, Rommy
datacite.creatorQuinones, John
datacite.creatorUlloa Rodriguez, Patricio
datacite.creatorVillalobos, Elias Figueroa
datacite.creatorBravo, Leon A.
datacite.creatorGraether, Steffen P.
datacite.creatorFarias, Jorge G.
datacite.date2024
datacite.subject.englishantifreeze proteins
datacite.subject.englishapoplastic extract
datacite.subject.englishice recrystallization inhibition
datacite.subject.englishthermal hysteresis
datacite.subject.englishcryoprotectant
datacite.titleNovel Apoplastic Antifreeze Proteins of Deschampsia antarctica as Enhancer of Common Cell Freezing Media for Cryobanking of Genetic Resources, a Preliminary Study
dc.date.accessioned2024-05-27T18:27:22Z
dc.date.available2024-05-27T18:27:22Z
dc.description.abstractAntifreeze proteins (AFPs) are natural biomolecules found in cold-adapted organisms that lower the freezing point of water, allowing survival in icy conditions. These proteins have the potential to improve cryopreservation techniques by enhancing the quality of genetic material postthaw. , a freezing-tolerant plant, possesses AFPs and is a promising candidate for cryopreservation applications. In this study, we investigated the cryoprotective properties of AFPs from extracts on Atlantic salmon spermatozoa. Apoplastic extracts were used to determine ice recrystallization inhibition (IRI), thermal hysteresis (TH) activities and ice crystal morphology. Spermatozoa were cryopreserved using a standard cryoprotectant medium (C+) and three alternative media supplemented with apoplastic extracts. Flow cytometry was employed to measure plasma membrane integrity (PMI) and mitochondrial membrane potential (MMP) postthaw. Results showed that a low concentration of AFPs (0.05 mg/mL) provided significant IRI activity. Apoplastic extracts from demonstrated a cryoprotective effect on salmon spermatozoa, with PMI comparable to the standard medium. Moreover, samples treated with apoplastic extracts exhibited a higher percentage of cells with high MMP. These findings represent the first and preliminary report that suggests that AFPs derived from apoplastic extracts of have the potential to serve as cryoprotectants and could allow the development of novel freezing media.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5783
dc.language.isoen
dc.publisherMDPI
dc.sourceBIOMOLECULES
oaire.resourceTypeArticle
uct.indizacionSCI
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