Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

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dc.contributor.authorMedina, Gustavo
dc.contributor.authorNeves, Patricia
dc.contributor.authorFlores-Martin, Sandra
dc.contributor.authorManosalva, Carolina
dc.contributor.authorAndaur, Marcela
dc.contributor.authorOtth, Carola
dc.contributor.authorLincopan, Nilton
dc.contributor.authorFernandez, Heriberto
dc.coverageChileen_US
dc.date2019-10
dc.date.accessioned2024-03-11T16:10:04Z
dc.date.available2024-03-11T16:10:04Z
dc.description.abstractArcobacter butzleri is an emerging foodborne zoonotic pathogen that has been isolated from environmental water sources. This pathogen establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba found in environmental matrices such as soil and water. The principal aim of this study was to analyse the transcriptional pattern of flagellar (flaA-flaB-flgH-motA) and other putative virulence genes (ciaB-cadF-mviN-pldA) of A. butzleri during its interaction with A. castellanii by quantitative real-time PCR. The transcriptional analysis showed up-regulation of all genes analysed before A. butzleri became established as an endocytobiont of A. castellanii. In contrast, while A. butzleri remains an endocytobiont, a significant and sustained decrease in the transcription of all analysed genes was observed. Our findings suggest that A. butzleri requires a biphasic transcriptional pattern of flagellar and other putative virulence genes to establish an endosymbiotic relationship with A. castellanii.en_US
dc.description.versionpostprinten_US
dc.formatPDFen_US
dc.identifier.citationARCHIVES OF MICROBIOLOGY, Vol. 201, Nº 8, 1075-1083, 2019en_US
dc.identifier.doi10.1007/s00203-019-01678-0en_US
dc.identifier.issn0302-8933(impresa); 1432-072X (electrónica)en_US
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5516
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rightsTodos los derechos reservados en_US
dc.sourceRevista ARCHIVES OF MICROBIOLOGYen_US
dc.subjectArcobacteren_US
dc.subjectFlagellar genes
dc.subjectEndosymbiosis
dc.subjectAcanthamoeba
dc.titleTranscriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellaniien_US
dc.typeArtículo de Revistaen_US
uct.carreraTecnología Médicaen_US
uct.catalogadorJCCLen_US
uct.comunidadCiencias de la Saluden_US
uct.disciplinaTecnología Médicaen_US
uct.facultadFacultad de Ciencias de la Saluden_US
uct.indizacionSCI EXPANDEDen_US
uct.indizacionEBSCO
uct.indizacionGoogle Scholar
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