Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis

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dc.contributor.authorRomero-Romero, Jesus L.; Inostroza-Blancheteau, Claudio; Orellana, Daniela; Aquea, Felipe; Reyes-Diaz, Marjorie; Gil, Pilar M.; Pablo Matte, Juan; Arce-Johnson, Patricio
dc.contributor.authorRomero Romero, Jesús
dc.contributor.authorInostroza Blancheteau, Claudio
dc.contributor.authorOrellana, Daniela
dc.contributor.authorAquea, Felipe
dc.contributor.authorReyes Diaz, Marjorie
dc.contributor.authorGil, Pilar M.
dc.contributor.authorMatte, Juan Pablo
dc.contributor.authorArce Johson, Patricio
dc.date2018
dc.date.accessioned2020-07-07T15:40:23Z
dc.date.available2020-07-07T15:40:23Z
dc.description.abstractWater-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB15, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospisen_US
dc.formatPDFen_US
dc.identifier.citationPlant Physiology and Biochemistry, Vol.130, 54-60en_US
dc.identifier.doi10.1016/j.plaphy.2018.06.034en_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2261
dc.language.isoenen_US
dc.rightsObra bajo licencia Creative Commons 3.0en_US
dc.sourcePlant Physiology and Biochemistryen_US
dc.subjectEstrés abióticoen_US
dc.subjectArabidopsisen_US
dc.subjectCisgénicoen_US
dc.subjectCitrus sinensisen_US
dc.titleStomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsisen_US
dc.typeArtículo de Revistaen_US
uct.carreraIngeniería en Recursos Naturales Renovablesen_US
uct.catalogadormlmen_US
uct.comunidadRecursos Naturalesen_US
uct.disciplinaCiencias de las Plantasen_US
uct.facultadFacultad de Recursos Naturalesen_US
uct.indizacionSCOPUSen_US
uct.nucleosNúcleo en Producción Alimentariaen_US
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