Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch

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datacite.alternateIdentifier.citationLatin American Journal of Aquatic Research, Vol. 47, N° 5, 860-864, 2019en_US
datacite.alternateIdentifier.doi10.3856/vol47-issue5-fulltext-16en_US
datacite.creatorMárquez Rodríguez, Lorenzo
datacite.creatorHernández Arias, Adrián
datacite.creatorCarvalho, Edison S.M
datacite.creatorMorales, Gabriel Alejandro
datacite.creatorDantagnan Dantagnan, Patricio
datacite.creatorDíaz López, Manuel
datacite.creatorMoyano López, Francisco
datacite.date2019
datacite.subjectOncorhynchus kisutchen_US
datacite.subjectCoho salmonen_US
datacite.subjectGastric proteasesen_US
datacite.subjectEnzymatic activityen_US
datacite.subjectpHen_US
datacite.subjectIonic strengthen_US
datacite.subjectSalmón plateadoen_US
datacite.subjectProteasas gástricasen_US
datacite.subjectActividad enzimaticaen_US
datacite.subjectFuerza iónicaen_US
datacite.titleEffects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutchen_US
dc.coverageChileen_US
dc.date.accessioned2022-10-21T16:25:38Z
dc.date.available2022-10-21T16:25:38Z
dc.description.abstractThe activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.en_US
dc.formatPDFen_US
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4811
dc.language.isoenen_US
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsObra bajo licencia Creative Commons Atribución-NoComercial 4.0 Internacionalen_US
dc.sourceLatin American Journal of Aquatic Research (Valparaíso)en_US
oaire.resourceTypeArtículo de Revistaen_US
uct.catalogadorbmcen_US
uct.comunidadRecursos Naturalesen_US
uct.facultadFacultad de Recursos Naturalesen_US
uct.indizacionSCIELOen_US
uct.indizacionScopus
uct.indizacionLatindex
uct.nucleosNúcleo en Producción Alimentariaen_US
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