The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)

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dc.contributor.authorBeltran Lissabet, Jorge Felix
dc.contributor.authorHerrera Belen, Lisandra
dc.contributor.authorLee Estevez, Manuel
dc.contributor.authorRisopatron, Jennie
dc.contributor.authorValdebenito Isler, Iván
dc.contributor.authorFigueroa Villalobos, Elías
dc.contributor.authorFarias, Jorge G.
dc.date2020
dc.date.accessioned2021-04-30T16:47:48Z
dc.date.available2021-04-30T16:47:48Z
dc.description.abstractAmong all the Ca2+ channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process. In fish sperm, the calcium channel plays a key role in sperm activation. However, the functionality of the CatSper channels has not been studied in any of the fish species. For the first time, we studied the relationship of the CatSper channel with sperm motility in a fish, using Atlantic salmon (Salmo salar) as the model. The results of our study showed that the CatSper channel in Salmo salar has chemical-physical characteristics similar to those reported for mammalian CatSper channels. In this work, it was shown that Salmo salar CatSper 3 protein has a molecular weight of approximately 55-kDa similar to Homo sapiens CatSper 3. In silico analyses suggest that this channel forms a heterotetramer sensitive to the specific inhibitor HC-056456, with a binding site in the center of the pore of the CatSper channel, hindering or preventing the influx of Ca (2+) ions. The in vitro assay of the sperm motility inhibition of Salmo salar with the inhibitor HC-056456 showed that sperm treated with this inhibitor significantly reduced the total and progressive motility (p < .0001), demonstrating the importance of this ionic channel for this cell. The complementation of the in silico and in vitro analyses of the present work demonstrates that the CatSper channel plays a key role in the regulation of sperm motility in Atlantic salmon.
dc.identifier.citationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,Vol.241,,2020
dc.identifier.doi10.1016/j.cbpa.2019.110634
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3535
dc.language.isoen
dc.publisherELSEVIER SCIENCE INC
dc.sourceCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
dc.subject.englishCatSper
dc.subject.englishSalmo salar
dc.subject.englishMotility
dc.subject.englishSperm
dc.titleThe CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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