Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines

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datacite.alternateIdentifier.citationINTERNATIONAL JOURNAL OF MORPHOLOGY,Vol.33,638-646,2015
datacite.creatorIli, Carmen G.
datacite.creatorBrebi, Priscilla
datacite.creatorGarcia, Patricia
datacite.creatorLeal, Pamela
datacite.creatorLopez, Jaime
datacite.creatorTapia, Oscar
datacite.creatorLetelier, Pablo
datacite.creatorWeber, Helga
datacite.creatorCastillo, Jonathan
datacite.creatorRoa, Juan C.
datacite.date2015
datacite.subject.englishApoptosis
datacite.subject.englishProliferation
datacite.subject.englishCervical cancer
datacite.subject.englishsiRNA
datacite.subject.englishc-FLIPL
datacite.titleEffects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
dc.date.accessioned2021-04-30T16:47:49Z
dc.date.available2021-04-30T16:47:49Z
dc.description.abstractOverexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P<0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P<0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P<0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3558
dc.language.isoen
dc.publisherSOC CHILENA ANATOMIA
dc.sourceINTERNATIONAL JOURNAL OF MORPHOLOGY
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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