Post-thaw dilution of Rhamdia quelen sperm improves the reproductive success

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dc.contributor.authorFranca, Thales de Souza
dc.contributor.authorGomes, Itamar Cossina
dc.contributor.authorSanches, Eduardo Antonio
dc.contributor.authorAtehortua, Maritza Perez
dc.contributor.authorTeixeira, Nathalia Santos
dc.contributor.authorRodrigues, Romulo Batista
dc.contributor.authorde Freitas, Thaiza Rodrigues
dc.contributor.authorGaluppo, Andrea Giannotti
dc.contributor.authorQuirino, Monike
dc.contributor.authorBenato, Jhony Lisboa
dc.contributor.authorMachado, Thales Lysakowski Flores
dc.contributor.authorMarques, Lis Santos
dc.contributor.authorBustamante Filho, Ivan Cunha
dc.contributor.authorBortolozzo, Fernando Pandolfo
dc.contributor.authorStreit Jr, Danilo Pedro
dc.date2022
dc.date.accessioned2022-08-01T18:52:49Z
dc.date.available2022-08-01T18:52:49Z
dc.description.abstractThe aim was to evaluate the effect of a post-thaw dilution of Rhamdia quelen sperm in 1.1% NaCl (325 mOsm kg(-1); pH 7.6; 24 degrees C) solution on the quality and reproductive capacity. Sperm from eight males were cryopreservation in nitrogen vapor at -170 degrees C for 18 h in 0.25 mL straws in a freezing medium containing 5% fructose, 5% Powdered milk, and 10% methanol. The samples were thawed and post-thaw diluted (1:20) in NaCl solution or not (control). The higher spermatozoa velocities were observed in the post-thaw diluted samples (curvilinear (VCL) -69 +/- 11 pm s(-1); average path (VAP) -45 +/- 8 pm s(-1); straight-line (VSL) -43 +/- 8 pm s(-1)) compared to the control (VCL -47 +/- 10 pm s(-1); VAP -31 +/- 6 pm s(-1); VSL -30 +/- 6 pm s(-1)). Greater straightness (STR), progression (PROG), and beat cross frequency (BCF) were observed in the post-thaw diluted samples (STR -96 +/- 7%; PROG -666 +/- 128 pm; BCF -42 +/- 2 Hz) than in control (STR -95 +/- 5%; PROG -463 +/- 92 pm; BCF -40 +/- 2 Hz). The strongly curled tail was the only morphology change that differ between the post-thaw diluted (5 +/- 2%) and control (2 +/- 1%). Membrane integrity, mitochondrial activity, and normal larvae rate were not different between treatments. Fertilization and hatching were higher in the post-thaw diluted sperm (93 +/- 3%; 82 +/- 9%) when compared to control samples (65 +/- 13%; 55 +/- 17%). Were used oocytes from one female, limiting these results. The post-thaw dilution improved the sperm kinetics and reproductive parameters. Thus, this methodology can be included in the sperm cryopreservation protocol for R. quelen.
dc.identifier.citationANIMAL REPRODUCTION SCIENCE,Vol.243,2022
dc.identifier.doi10.1016/j.anireprosci.2022.107018
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4633
dc.language.isoen
dc.publisherELSEVIER
dc.sourceANIMAL REPRODUCTION SCIENCE
dc.subject.englishSperm quality
dc.subject.englishSperm cryopreservation
dc.subject.englishSouth American silver catfish
dc.subject.englishArtificial fertilization
dc.subject.englishHatching
dc.subject.englishSperm dilution
dc.titlePost-thaw dilution of Rhamdia quelen sperm improves the reproductive success
dc.typeArticle
uct.indizacionSCI
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