Post-thaw dilution of Rhamdia quelen sperm improves the reproductive success

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datacite.alternateIdentifier.citationANIMAL REPRODUCTION SCIENCE,Vol.243,2022
datacite.alternateIdentifier.doi10.1016/j.anireprosci.2022.107018
datacite.creatorFranca, Thales de Souza
datacite.creatorGomes, Itamar Cossina
datacite.creatorSanches, Eduardo Antonio
datacite.creatorAtehortua, Maritza Perez
datacite.creatorTeixeira, Nathalia Santos
datacite.creatorRodrigues, Romulo Batista
datacite.creatorde Freitas, Thaiza Rodrigues
datacite.creatorGaluppo, Andrea Giannotti
datacite.creatorQuirino, Monike
datacite.creatorBenato, Jhony Lisboa
datacite.creatorMachado, Thales Lysakowski Flores
datacite.creatorMarques, Lis Santos
datacite.creatorBustamante Filho, Ivan Cunha
datacite.creatorBortolozzo, Fernando Pandolfo
datacite.creatorStreit Jr, Danilo Pedro
datacite.date2022
datacite.subject.englishSperm quality
datacite.subject.englishSperm cryopreservation
datacite.subject.englishSouth American silver catfish
datacite.subject.englishArtificial fertilization
datacite.subject.englishHatching
datacite.subject.englishSperm dilution
datacite.titlePost-thaw dilution of Rhamdia quelen sperm improves the reproductive success
dc.date.accessioned2022-08-01T18:52:49Z
dc.date.available2022-08-01T18:52:49Z
dc.description.abstractThe aim was to evaluate the effect of a post-thaw dilution of Rhamdia quelen sperm in 1.1% NaCl (325 mOsm kg(-1); pH 7.6; 24 degrees C) solution on the quality and reproductive capacity. Sperm from eight males were cryopreservation in nitrogen vapor at -170 degrees C for 18 h in 0.25 mL straws in a freezing medium containing 5% fructose, 5% Powdered milk, and 10% methanol. The samples were thawed and post-thaw diluted (1:20) in NaCl solution or not (control). The higher spermatozoa velocities were observed in the post-thaw diluted samples (curvilinear (VCL) -69 +/- 11 pm s(-1); average path (VAP) -45 +/- 8 pm s(-1); straight-line (VSL) -43 +/- 8 pm s(-1)) compared to the control (VCL -47 +/- 10 pm s(-1); VAP -31 +/- 6 pm s(-1); VSL -30 +/- 6 pm s(-1)). Greater straightness (STR), progression (PROG), and beat cross frequency (BCF) were observed in the post-thaw diluted samples (STR -96 +/- 7%; PROG -666 +/- 128 pm; BCF -42 +/- 2 Hz) than in control (STR -95 +/- 5%; PROG -463 +/- 92 pm; BCF -40 +/- 2 Hz). The strongly curled tail was the only morphology change that differ between the post-thaw diluted (5 +/- 2%) and control (2 +/- 1%). Membrane integrity, mitochondrial activity, and normal larvae rate were not different between treatments. Fertilization and hatching were higher in the post-thaw diluted sperm (93 +/- 3%; 82 +/- 9%) when compared to control samples (65 +/- 13%; 55 +/- 17%). Were used oocytes from one female, limiting these results. The post-thaw dilution improved the sperm kinetics and reproductive parameters. Thus, this methodology can be included in the sperm cryopreservation protocol for R. quelen.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4633
dc.language.isoen
dc.publisherELSEVIER
dc.sourceANIMAL REPRODUCTION SCIENCE
oaire.resourceTypeArticle
uct.indizacionSCI
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