Molecularly Imprinted Nanoparticles Assay (MINA) in Pseudo ELISA: An Alternative to Detect and Quantify Octopamine in Water and Human Urine Samples

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datacite.alternateIdentifier.citationPOLYMERS,Vol.11,,2019
datacite.alternateIdentifier.doi10.3390/polym11091497
datacite.creatorMoczko, Ewa
datacite.creatorDiaz, Richard
datacite.creatorRivas, Bernabe
datacite.creatorGarcia, Camilo
datacite.creatorPereira, Eduardo
datacite.creatorPiletsky, Sergey
datacite.creatorCaceres, Cesar
datacite.date2019
datacite.subject.englishmolecularly imprinted nanoparticles assay
datacite.subject.englishmolecularly imprinted polymers
datacite.subject.englishELISA
datacite.subject.englishdoping
datacite.subject.englishoctopamine
datacite.titleMolecularly Imprinted Nanoparticles Assay (MINA) in Pseudo ELISA: An Alternative to Detect and Quantify Octopamine in Water and Human Urine Samples
dc.date.accessioned2021-04-30T16:59:15Z
dc.date.available2021-04-30T16:59:15Z
dc.description.abstractIn 2004, octopamine was added to the list of drugs banned by the world anti-doping agency (WADA) and prohibited in any sport competition. This work aims to develop a new analytical method to detect octopamine in water and human urine samples. We proposed a pseudo-enzyme-linked immunosorbent assay (pseudo-ELISA) by replacing traditional monoclonal antibodies with molecularly imprinted polymer nanoparticles (nanoMIPs). NanoMIPs were synthesised by a solid-phase approach using a persulfate initiated polymerisation in water. Their performance was analysed in pseudo competitive ELISA based on the competition between free octopamine and octopamine-HRP conjugated. The final assay was able to detect octopamine in water within the range 1 nmol.L-1-0.1 mol.L-1 with a detection limit of 0.047 +/- 0.00231 mu g.mL(-1) and in human urine samples within the range 1 nmol.L-1-0.0001 mol.L-1 with a detection limit of 0.059 +/- 0.00281 mu g.mL(-1). In all experiments, nanoMIPs presented high affinity to the target molecules and almost no cross-reactivity with analogues of octopamine such as pseudophedrine or l-Tyrosine. Only slight interference was observed from the human urine matrix. The high affinity and specificity of nanoMIPs and no need to maintain a cold chain logistics makes the nanoMIPs a competitive alternative to antibodies. Furthermore, this work is the first attempt to use nanoMIPs in pseudo-ELISA assays to detect octopamine.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3776
dc.language.isoen
dc.publisherMDPI
dc.sourcePOLYMERS
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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