High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor

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dc.contributor.authorVidal Seguel, Nicolas
dc.contributor.authorCabrera, Camilo
dc.contributor.authorFerrada, Luciano
dc.contributor.authorArtigas Arias, Macarena
dc.contributor.authorAlegria Molina, Andrea
dc.contributor.authorSanhueza, Sergio
dc.contributor.authorFlores, Alejandra
dc.contributor.authorHuard, Nolberto
dc.contributor.authorSapunar, Jorge
dc.contributor.authorSalazar, Luis A.
dc.contributor.authorMcGregor, Reuben
dc.contributor.authorNova Lamperti, Estefania
dc.contributor.authorMarzuca Nassr, Gabriel Nasri
dc.date2023
dc.date.accessioned2024-05-27T18:27:53Z
dc.date.available2024-05-27T18:27:53Z
dc.description.abstractNeutrophil extracellular trap formation (NETosis) is a mechanism used by neutrophils to capture pathogens with their own DNA. However, the exacerbation of this immune response is related to serious inflammatory diseases. Aging is known to lead to an excessive increase in NETosis associated with various diseases. Under this scenario, the search for strategies that regulate the release of NETosis in older people becomes relevant. High-intensity interval training (HIIT) involves repeated bouts of relatively intense exercise with alternating short recovery periods. This training has shown beneficial effects on health parameters during aging and disease. However, little is known about the potential role of HIIT in the regulation of NETosis in healthy older people. The aim of this study was to evaluate the induction of NETosis by serum from healthy young and older men, before and after 12 weeks of HIIT using healthy neutrophils as a biosensor. HIIT was performed 3 times per week for 12 weeks in young (YOUNG; 21 +/- 1 years, BMI 26.01 +/- 2.64 kg center dot m(-2), n = 10) and older men (OLDER; 66 +/- 5 years, BMI 27.43 +/- 3.11 kg center dot m(-2), n = 10). Serum samples were taken before and after the HIIT program and NETosis was measured with live cell imaging in donated neutrophils cultured with serum from the participants for 30 h. Our results showed that serum from older men at baseline induced greater baseline NETosis than younger men (p < 0.05; effect size, >= 0.8), and 12 weeks of HIIT significantly reduced (Interaction Effect, p < 0.05; effect size, 0.134) the induction of NETosis in older men. In conclusion, HIIT is a feasible non-invasive training strategy modulating NETosis induction. Additionally, the use of neutrophils as a biosensor is an effective method for the quantification of NETosis induction in real time.
dc.identifier.citationEXPERIMENTAL GERONTOLOGY,Vol.181,2023
dc.identifier.doi10.1016/j.exger.2023.112280
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5908
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.sourceEXPERIMENTAL GERONTOLOGY
dc.subject.englishNETs
dc.subject.englishNeutrophil extracellular trap
dc.subject.englishHigh-intensity interval training
dc.subject.englishExercise
dc.subject.englishElderly
dc.titleHigh-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor
dc.typeArticle
uct.indizacionSCI
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