Morphometric of blastomeres in Salmo salar

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dc.contributor.authorEffer Roldan, Brian
dc.contributor.authorSanchez, Ruben R.
dc.contributor.authorUbilla Madrid, Andrea
dc.contributor.authorFigueroa Villalobos, Elías
dc.contributor.authorValdebenito Isler, Iván
dc.date2014
dc.date.accessioned2021-04-30T16:28:44Z
dc.date.available2021-04-30T16:28:44Z
dc.description.abstractFor Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of S. salar. From a pool of eggs incubated at 7.5 degrees C, 100 microphotographs of blastodiscs were extracted and analyzed at different incubation periods: 12, 14, 16, 20 or 24 h. Blastodiscs were characterized morphologically after 16, 20 or 24 h incubation, and classified into symmetric or asymmetric groups according to their morphology. The ratio of length (L) versus width (W) of each blastomere was determined, to establish its symmetry. In addition, 20 microphotographs of blastodiscs of normal appearance were analysed morphologically (control blastodisc: CB) for comparison (20 or 24 h). Results show that the first cleavage ends after 16 h of development. Seven categories were established during blastomere characterization: 47% normal (G1); 27% with dispersed margins (G2); 10% unequal (G3); 9% 'pie-shaped' (G4); 3% amorphous (G5); 2% three equal blastomeres and one different one (G6); and 2% with eccentric cleavage (G7). Although the incidence of abnormal cleavage in S. salar is uncertain, there is a potential for some asymmetries to be corrected during embryogenesis to generate viable individuals. More studies are necessary to correlate these abnormal cleavage patterns with indicators of quality in the later stages of embryogenesis in this species, to establish a quality assessment tool for gametes and/or embryos in salmonid species.
dc.identifier.citationZYGOTE,Vol.22,470-475,2014
dc.identifier.doi10.1017/S0967199412000718
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2649
dc.language.isoen
dc.publisherCAMBRIDGE UNIV PRESS
dc.sourceZYGOTE
dc.subject.englishBlastomeres
dc.subject.englishEmbryos
dc.subject.englishMorphometric
dc.subject.englishSalmo salar
dc.titleMorphometric of blastomeres in Salmo salar
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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