Effects of leptin administration on development, vascularization and function of Corpus luteum in alpacas submitted to pre-ovulatory fasting

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dc.contributor.authorCecilia Norarnbuena, Maria
dc.contributor.authorHernandez, Francisca
dc.contributor.authorMaureira, Jonathan
dc.contributor.authorRubilar, Carolina
dc.contributor.authorAlfaro, Jorge
dc.contributor.authorSilva, Gonzalo
dc.contributor.authorSilva Jiménez, Mauricio
dc.contributor.authorUlloa-Leal, Cesar
dc.date2017
dc.date.accessioned2021-04-30T16:32:56Z
dc.date.available2021-04-30T16:32:56Z
dc.description.abstractThe objective of this study was to determine the effect of leptin administration on the development, vascularization and function of Corpus luteum (CL) in alpacas submitted to pre-ovulatory fasting. Fourteen alpacas were kept in fasting conditions for 72 h and received five doses of o-leptin (2 mu g/kg e.v.; Leptin group) or saline (Control group) every 12 h. Ovulation was induced with a GnRH dose (Day 0). The ovaries were examined every other day by trans-rectal ultrasonography (7.5 MHz; mode B and power Doppler) from Day 0 to 13 to determine the pre-ovulatory follicle diameter and ovulation, and then to monitor CL diameter and vascularization until the regression phase. Serial blood samples were taken after GnRH treatment to determine plasma LH concentration; and every other day from Days 1 to 13 to determine plasma progesterone and leptin concentrations. The pre-ovulatory follicle and CL diameter, LH, progesterone and leptin plasma concentrations were not affected by treatment (P > 0.05). The vascularization area of the CL was, nevertheless, affected by the treatment (P < 0.01) with significant differences between groups at Days 3, 7 and 9 (P < 0.05). The Leptin group had a larger maximum vascularization area (0.67 +/- 0.1 compared with 0.35 +/- 0.1 cm(2); P < 0.05). In addition, there was a positive correlation between CL vascularization, CL diameter and plasma progesterone. The exogenous administration of leptin during pre-ovulatory fasting increased the vascularization of the CL in alpacas in vivo.
dc.identifier.citationANIMAL REPRODUCTION SCIENCE,Vol.182,28-34,2017
dc.identifier.doi10.1016/j.anireprosci.2017.04.006
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2938
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.sourceANIMAL REPRODUCTION SCIENCE
dc.subject.englishLeptin
dc.subject.englishCorpus luteum
dc.subject.englishVascularization
dc.subject.englishCamelids
dc.subject.englishFasting
dc.titleEffects of leptin administration on development, vascularization and function of Corpus luteum in alpacas submitted to pre-ovulatory fasting
dc.typeArticle
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uct.indizacionSCI
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