Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation

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dc.contributor.authorDíaz, R.
dc.contributor.authorLee Estévez, M.
dc.contributor.authorQuiñoñes, J.
dc.contributor.authorDumorné, K.
dc.contributor.authorShort, S.
dc.contributor.authorUlloa Rodríguez, P.
dc.contributor.authorValdebenito Isler, Iván
dc.contributor.authorSepúlveda, N.
dc.contributor.authorFarías, J.
dc.date2019
dc.date.accessioned2020-07-29T00:17:49Z
dc.date.available2020-07-29T00:17:49Z
dc.description.abstractThe cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to evaluate the morphology and lipid composition of Atlantic salmon (Salmo salar) spermatozoa after storage at 4 °C and cryopreservation. Semen samples were obtained by stripping. One aliquot was stored at 4 °C for 7 days, and another aliquot was cryopreserved. The morphology and ultrastructure were analysed using electron microscopy. The lipid composition was analysed by gas chromatography and a commercial kit. After cold storage, the mitochondrion was the most affected component; however, plasma membrane rupture and detachment of the flagellum were also observed. Morphological abnormalities were greater in cryopreserved spermatozoa. The head and mid-piece were dehydrated, sperm membranes were vesiculated, and alterations of mitochondria were observed. After cold storage and cryopreservation, there were less polyunsaturated and omega-3 fatty acids. Furthermore, there was an increase in saturated fatty acids and decrease in cholesterol concentration after cryopreservation (P < 0.05). Based on the results, cryopreservation drastically damaged sperm membranes; the cryogenic damage was associated with membrane lipid composition alterations. The sperm membranes were affected less by cold storage but there was also a decrease of some lipids; therefore, there is a need for improvement in cold storage processes to decrease structural damage of spermatozoa so that semen cryopreservation can be effectively used in the salmon industryen_US
dc.formatPDFen_US
dc.identifier.citationAnimal Reproduction Science, Vol.204, 50-59, 2019en_US
dc.identifier.doi10.1016/j.anireprosci.2019.03.004en_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2278
dc.language.isoenen_US
dc.sourceAnimal Reproduction Scienceen_US
dc.subjectColesterolen_US
dc.subjectÁcidos grasosen_US
dc.subjectSalmo salaren_US
dc.subjectCriopreservaciónen_US
dc.subjectEspermatozoides de pescadoen_US
dc.titleChanges in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservationen_US
dc.typeArtículo de Revistaen_US
uct.catalogadorpopen_US
uct.comunidadRecursos Naturalesen_US
uct.indizacionISI - Science Citation Indexen_US
uct.indizacionSCOPUSen_US
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