Role of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)

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datacite.alternateIdentifier.citationAQUACULTURE,Vol.538,,2021
datacite.alternateIdentifier.doi10.1016/j.aquaculture.2021.736537
datacite.creatorBeltran, Jorge F.
datacite.creatorFigueiras-Fierro, D.
datacite.creatorZamorano, Mauricio
datacite.creatorHerrera Belen, Lisandra
datacite.creatorRisopatron, Jennie
datacite.creatorValdebenito, Ivan
datacite.creatorNorambuena, Juan-Alejandro
datacite.creatorFarias, Jorge G.
datacite.date2021
datacite.subject.englishSalmo salar
datacite.subject.englishMotility
datacite.subject.englishIn vitro
datacite.subject.englishIn silico
datacite.subject.englishChannel
datacite.subject.english
datacite.titleRole of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)
dc.date.accessioned2021-10-04T18:54:33Z
dc.date.available2021-10-04T18:54:33Z
dc.description.abstractSalmonid sperm motility is regulated by different factors, such as ion concentration, osmotic pressure, pH, and temperature. The Ca2+ cation-dependent regulation and Ca2+-binding proteins are key regulatory mechanisms in the sperm activation process. In this study, the function of transient receptor potential ankyrin 1, transient receptor potential cation channel subfamily V member 4, and Na+/Ca2+ exchanger 1 on the sperm motility of Salmo salar were studied in vitro and in silico. Sperm inhibition assay results showed that the inhibition of the Na+/Ca2+ exchanger produced a significant decrease in total and progressive motility (p < 0.0001). The molecular docking-based analysis showed that inhibitor KB-R7943 binds strongly (-6.0 kcal/mol) in the Ca2+binding region in this exchanger. Additionally, it was demonstrated that, in contrast to Na+/Ca2+ exchange, neither transient receptor potential ankyrin 1 nor transient receptor potential cation channel subfamily V member 4 was directly associated with sperm motility. This research provides new and useful insights into the role of Ca2+-binding proteins present in the plasma membrane that regulate the sperm motility of Salmo salar.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/4358
dc.language.isoen
dc.publisherELSEVIER
dc.sourceAQUACULTURE
oaire.resourceTypeArticle
uct.indizacionSCI
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