Intra‐amoebic localization of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

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dc.contributor.authorMedina, Gustavo
dc.contributor.authorLeyan, Pilar
dc.contributor.authorViera da Silva, C.
dc.contributor.authorFlores-Martin, Sandra
dc.contributor.authorManosalva, Carolina
dc.contributor.authorHernadez, Heriberto
dc.coverageChileen_US
dc.date2019-12
dc.date.accessioned2024-03-18T21:15:53Z
dc.date.available2024-03-18T21:15:53Z
dc.description.abstractAcanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing their association with endoplasmic reticulum vesicles and mitochondria. Through confocal microscopy, we observed that during the early stages of endosymbiosis, there is not colocalization between amoebic vacuoles containing A. butzleri and mitochondria or ER vesicles of A. castellanii. Considering that energy production of this bacterium occurs via metabolism of amino acids or the tricarboxylic acid cycle, these results contribute to explain the absence of bacterial replication, since A. butzleri would not have access to the nutrients found in endoplasmic reticulum vesicles and mitochondria. In addition, we observe that A. butzleri induces significantly the actin polymerization of A. castellanii during the early stages of endosymbiosis.en_US
dc.description.versionpostprinten_US
dc.formatPDFen_US
dc.identifier.citationARCHIVES OF MICROBIOLOGY, Vol. 201, N° 10, 1447-1452, 2019en_US
dc.identifier.doi10.1007/s00203-019-01699-9en_US
dc.identifier.issn1432-072X (electrónica)en_US
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5553
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rightsTodos los derechos reservados
dc.sourceARCHIVES OF MICROBIOLOGYen_US
dc.subjectAcanthamoebaen_US
dc.subjectEndosymbiosis
dc.subjectArcobacter
dc.subjectConfocal microscopy
dc.titleIntra‐amoebic localization of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellaniien_US
dc.typeArtículo de Revistaen_US
uct.carreraTecnología Médicaen_US
uct.catalogadorJCCLen_US
uct.comunidadCiencias de la Saluden_US
uct.disciplinaTecnología Médicaen_US
uct.facultadFacultad de Ciencias de la Saluden_US
uct.indizacionESCIen_US
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