CLI: A new protocol for the isolation of Lactic Acid Bacteria from complex plant samples

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datacite.alternateIdentifier.citationJOURNAL OF MICROBIOLOGICAL METHODS,Vol.221,2024
datacite.alternateIdentifier.doi10.1016/j.mimet.2024.106937
datacite.creatorChavez, Mariannys
datacite.creatorViscardi, Sharon
datacite.creatorRuiz, Maria Jose
datacite.creatorSans Serramitjana, Eulalia
datacite.creatorDuran, Paola
datacite.date2024
datacite.subject.englishNew method
datacite.subject.englishPlant probiotic
datacite.subject.englishComplex samples
datacite.subject.englishLactic Acid Bacteria
datacite.titleCLI: A new protocol for the isolation of Lactic Acid Bacteria from complex plant samples
dc.date.accessioned2024-09-10T18:47:13Z
dc.date.available2024-09-10T18:47:13Z
dc.description.abstractLactic Acid Bacteria (LAB) are predominantly probiotic microorganisms and the most are Generally Recognized As Safe (GRAS). LAB inhabit in the human gut ecosystem and are largely found in fermented foods and silage. In the last decades, LAB have also has been found in plant microbiota as a new class of microbes with probiotic activity to plants. For this reason, today the scientific interest in the study and isolation of LAB for agronomic application has increased. However, isolation protocols from complex samples such as plant tissues are scarce and inefficient. In this study, we developed a new protocol (CLI, Complex samples LAB Isolation) which yields purified LAB from plants. The sensitivity of CLI protocol was sufficient to isolate representative microorganisms of LAB genera (i.e. Leuconostoc, Lactococcus and Enterococcus). CLI protocol consists on five steps: i) sample preparation and pre-incubation in 1% sterile peptone at 30 degrees C for 24-48 h; ii) Sample homogenization in vortex by 10 min; iii) sample serial dilution in quarter-strength Ringer solution, iv) incubation in MRS agar plates with 0.2% of sorbic acid, with 1% of CaCO3, O-2 < 15%, at pH 5.8 and 37 degrees C for 48 h.; v) Selection of single colonies with LAB morphology and CaCO3-solubilization halo. Our scientific contribution is that CLI protocol could be used for several complex samples and represents a useful method for further studies involving native LAB.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6005
dc.language.isoen
dc.publisherELSEVIER
dc.sourceJOURNAL OF MICROBIOLOGICAL METHODS
oaire.resourceTypeArticle
uct.indizacionSCI
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