Novel microsatellite markers discovery in Patagonian toothfish (Dissostichus eleginoides) using high-throughput sequencing

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datacite.alternateIdentifier.citationMOLECULAR BIOLOGY REPORTS,Vol.46,5525-5530,2019
datacite.alternateIdentifier.doi10.1007/s11033-019-04912-6
datacite.creatorGarcia, Killen Ko
datacite.creatorTouma, Jorge
datacite.creatorBravo, Scarleth
datacite.creatorLeiva, Francisco
datacite.creatorVargas Chacoff, Luis
datacite.creatorValenzuela, Ariel
datacite.creatorDantagnan Dantagnan, Patricio
datacite.creatorAmthauer, Rodolfo
datacite.creatorReyes, Alberto
datacite.creatorVidal, Rodrigo
datacite.date2019
datacite.subject.englishToothfish
datacite.subject.englishDissostichus eleginoides
datacite.subject.englishSimple sequence repeat
datacite.subject.englishMicrosatellite
datacite.subject.englishHigh-throughput sequencing
datacite.titleNovel microsatellite markers discovery in Patagonian toothfish (Dissostichus eleginoides) using high-throughput sequencing
dc.date.accessioned2021-04-30T17:05:06Z
dc.date.available2021-04-30T17:05:06Z
dc.description.abstractPatagonian toohfish (Dissostichus eleginoides), is a sub Antartic notothenioid fish key in the marine ecosystem that sustains fishery of higher commercial value in the world. However, there are a scarce knowledge or information about its population genetic background, product of the almost null information of molecular markers available for this species. Here, we use high-throughput sequencing technology (Illumina platform) to develop 1071 microsatellite loci, of which 22 loci were selected to evaluation. Polymorphism and genetic diversity of each locus was assessed in two locations distant by 2370 km. Considering both locations, a mean PIC value of 0.748 was estimated. Selected microsatellite loci showed among two to seventeen alleles by locus in the first location and two to twelve in the second. The observed heterozygosity varied from 0.18 to 0.91 and from 0.12 to 0.87 for the first and second location, respectively. While, the expected heterozygosity ranged from 0.15 to 0.92 and from 0.11 to 0.90. Three loci were monomorphic in only one location. Microsatellite markers developed here will be useful in future studies on conservation, fishery and population genetics of this species.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3958
dc.language.isoen
dc.publisherSPRINGER
dc.sourceMOLECULAR BIOLOGY REPORTS
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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