Novel microsatellite markers discovery in Patagonian toothfish (Dissostichus eleginoides) using high-throughput sequencing

dc.contributor.authorGarcia, Killen Ko
dc.contributor.authorTouma, Jorge
dc.contributor.authorBravo, Scarleth
dc.contributor.authorLeiva, Francisco
dc.contributor.authorVargas Chacoff, Luis
dc.contributor.authorValenzuela, Ariel
dc.contributor.authorDantagnan Dantagnan, Patricio
dc.contributor.authorAmthauer, Rodolfo
dc.contributor.authorReyes, Alberto
dc.contributor.authorVidal, Rodrigo
dc.date2019
dc.date.accessioned2021-04-30T17:05:06Z
dc.date.available2021-04-30T17:05:06Z
dc.description.abstractPatagonian toohfish (Dissostichus eleginoides), is a sub Antartic notothenioid fish key in the marine ecosystem that sustains fishery of higher commercial value in the world. However, there are a scarce knowledge or information about its population genetic background, product of the almost null information of molecular markers available for this species. Here, we use high-throughput sequencing technology (Illumina platform) to develop 1071 microsatellite loci, of which 22 loci were selected to evaluation. Polymorphism and genetic diversity of each locus was assessed in two locations distant by 2370 km. Considering both locations, a mean PIC value of 0.748 was estimated. Selected microsatellite loci showed among two to seventeen alleles by locus in the first location and two to twelve in the second. The observed heterozygosity varied from 0.18 to 0.91 and from 0.12 to 0.87 for the first and second location, respectively. While, the expected heterozygosity ranged from 0.15 to 0.92 and from 0.11 to 0.90. Three loci were monomorphic in only one location. Microsatellite markers developed here will be useful in future studies on conservation, fishery and population genetics of this species.
dc.identifier.citationMOLECULAR BIOLOGY REPORTS,Vol.46,5525-5530,2019
dc.identifier.doi10.1007/s11033-019-04912-6
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3958
dc.language.isoen
dc.publisherSPRINGER
dc.sourceMOLECULAR BIOLOGY REPORTS
dc.subject.englishToothfish
dc.subject.englishDissostichus eleginoides
dc.subject.englishSimple sequence repeat
dc.subject.englishMicrosatellite
dc.subject.englishHigh-throughput sequencing
dc.titleNovel microsatellite markers discovery in Patagonian toothfish (Dissostichus eleginoides) using high-throughput sequencing
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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