Validation method for extraction and determination of marbofloxacin in plasma and edible chicken tissues

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datacite.alternateIdentifier.citationMalaysian Journal of Analytical Sciences, Vol. 22, N°2, 197-202, 2018en_US
datacite.alternateIdentifier.doi10.17576/mjas-2018-2202-04en_US
datacite.creatorUrzúa, Natalia Francisca
datacite.creatorPrieto, Guillermo Fermín
datacite.creatorLüders, Carlos Fernando
datacite.creatorTonini, María Paula
datacite.creatorPicco, Eduardo Jesús
datacite.creatorErrecalde, Carlos Alberto
datacite.date2018
datacite.subjectMarbofloxacinaen_US
datacite.subjectCromatografía líquida de alto rendimientoen_US
datacite.subjectPollosen_US
datacite.titleValidation method for extraction and determination of marbofloxacin in plasma and edible chicken tissuesen_US
datacite.titleKaedah validasi bagi pengesktrakan dan penentuan marbofloxacin di dalam plasma dan tisu ayam yang boleh di makan
dc.date.accessioned2020-04-15T00:05:08Z
dc.date.available2020-04-15T00:05:08Z
dc.description.abstractMarbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through highperformance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 499 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r2= 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickensen_US
dc.formatPDFen_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2161
dc.language.isoenen_US
dc.sourceMalaysian Journal of Analytical Sciencesen_US
oaire.resourceTypeArtículo de Revistaen_US
uct.catalogadorpopen_US
uct.comunidadRecursos Naturalesen_US
uct.indizacionSCOPUSen_US
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