Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

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dc.contributor.authorRuiz, Jaime
dc.contributor.authorSantayana, R. Paulo
dc.contributor.authorMendoza, M. Jose
dc.contributor.authorLandeo, J. Leandra
dc.contributor.authorHuaman, Elizabeth
dc.contributor.authorTicllacuri, Flamel
dc.contributor.authorMujica, L. Fidel
dc.contributor.authorSilva Jiménez, Mauricio
dc.contributor.authorRatto, Marcelo H.
dc.date2017
dc.date.accessioned2021-04-30T16:59:14Z
dc.date.available2021-04-30T16:59:14Z
dc.description.abstractWe evaluated the effect of in vitro maturation time, sperm selection and oxygen tension on alpaca embryo development. In Experiment I, Cumulus Oocyte-Complexes (COCs) were obtained from abattoir ovaries and in vitro matured in TCM-199 for 24 (n = 217), 28 (215), or 32 h (223) at 38.5 degrees C, high humidity and 5% CO2 in air. Oocytes from 24 (n = 392), 28 (n = 456) or 32 (n = 368) h groups were in vitro fertilized with epididymal sperm and cultured in SOFaa at 38.5 degrees C, high humidity and 5% CO2, 5% O-2 and 90% N-2 for 7 days. Embryo development was evaluated on Day 2, 5 and Day 7 of in vitro culture (Day 0 = in vitro fertilization). In Experiment II, a 2 by 2 -factorial design was used to determine the effect of sperm selection (Swim -up vs Percoll) and oxygen tension (20% vs 5%) during embryo culture and their interaction on embryo development. COCs were in vitro matured for 32 h at 38.5 degrees C and 5% CO2 in air and then in vitro inseminated with epididymal sperm processed by swim-up or Percoll. Zygotes were cultured in SOFaa + cumulus cells at 38.5 degrees C under 20 or 5% of O-2 tension and high humidity for 7 days. A total of 235, 235, 253 and 240 oocytes were assigned to: swim-up+20 O-2, swim-up+5 O-2 or Percoll+20 O-2, Percoll+5 O-2, groups respectively. The proportion of oocytes reaching MII stage was highest after 32 h of in vitro maturation (P < 0.05). Blastocyst rate (29.1 +/- 2.7%) was also highest for COCs matured for 32 h (Exp I). In Experiment II, Blastocysts rate (26.03 +/- 4.7; 27.7 +/- 43; 29.7 +/- 3.8 and 27.6 +/- 4.2% for swimup+20 O-2, swim-up+5 O-2 or Percoll+20 O-2, Percoll+5 O-2, respectively) was not affected by sperm selection method (P = 0.8), oxygen tension (P = 0.9) or their interaction (P = 0.5). (C) 2017 Elsevier Inc. All rights reserved.
dc.identifier.citationTHERIOGENOLOGY,Vol.95,127-132,2017
dc.identifier.doi10.1016/j.theriogenology.2017.03.006
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3763
dc.language.isoen
dc.publisherELSEVIER SCIENCE INC
dc.sourceTHERIOGENOLOGY
dc.subject.englishAlpaca
dc.subject.englishOocytes
dc.subject.englishEmbryos
dc.subject.englishSperm selection
dc.subject.englishOxygen tension
dc.titleEffect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas
dc.typeArticle
uct.catalogadorWOS
uct.indizacionSCI
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