Sexual and asexual reproductive aspects of Leontochir ovallei, a rare and endange red geophyte of the Atacama Desert

Simple item page
dc.contributor.authorVargas, Marta
dc.contributor.authorJofre, Elda
dc.contributor.authorNavarrete, Carlos
dc.contributor.authorBravo, Jaime
dc.contributor.authorJamett, Fabiola
dc.contributor.authorInostroza Blancheteau, Claudio
dc.contributor.authorIbañez, Cristian
dc.date2018
dc.date.accessioned2020-07-07T14:25:43Z
dc.date.available2020-07-07T14:25:43Z
dc.description.abstractBackground: "Garra de Leon" (Leontochir ovallei) is an ephemeral endangered Alstroemeriaceae species endemic to Chile. Despite many efforts to improve the conservation of this species, the stimulation of dormant seeds and the production of rhizomes under controlled conditions remain unexplored. The aims of this study were to examine the germination responses of L. ovallei seeds under different in vitro conditions and to evaluate the formation of viable rhizomes after transplantation from in vitro to ex vitro conditions. Methods: We evaluated five in vitro seed germination treatments: (1) acid scarification, (2) acid scarification followed by imbibition of seeds in aerated water, (3) imbibition of seeds in gibberellic acid, (4) clipping of seeds with a scalpel, and (5) seeds without any treatment (control). Seedlings obtained under in vitro conditions were transplanted to ex vitro conditions following a gradual acclimation process. After eight months, the number of rhizomes per plant was counted. To test asexual multiplication, each rhizome with its respective storage organ was divided using a scalpel and then left to rest for two years before subsequent evaluation of viability. After that period, the rhizomes were re-hydrated, and the emergence of plants after three months was evaluated. Results: Seeds exposed to treatment 2 showed the highest germination percentage (36%), followed by the seeds whose coats were clipped (14%) and seeds treated with sulphuric acid (8%). The seed germination of the control treatment was 3%. After transplantation to ex vitro conditions, we obtained 220 rhizomes, with an average of three to six rhizomes per plant. After two years of dormancy, a total of 34 rhizomes (of 220 rhizomes) reactivated growth after re-ydration, indicating that, at least, 15,5% responded positively to watering. Conclusions: We found that the seed germination of L. ovallei was increased by strong acidic conditions, suggesting that the main type of seed dormancy in this species is physical. However, germination was further increased by aeration, indicating embryo dormancy. In addition, we were able to obtain viable rhizomes by transplanting seedlings from in vitro to ex vitro conditions, which may be helpful for the propagation and ex situ conservation of this rare geophyte of Chile and potentially other geophyte species adapted to wet-dry cycle environmentsen_US
dc.formatPDFen_US
dc.identifier.citationRevista Chilena de Historia Natural. Vol.91, N°1en_US
dc.identifier.doi10.1186/s40693-018-0075-8en_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2257
dc.language.isoenen_US
dc.rightsObra bajo licencia Creative Commons 3.0en_US
dc.sourceRevista Chilena de Historia Naturalen_US
dc.subjectAlstroemeriaceaeen_US
dc.subjectDesierto Atacama-Chileen_US
dc.subjectGerminación de Semillas in Vitroen_US
dc.subjectProducción de rizomasen_US
dc.titleSexual and asexual reproductive aspects of Leontochir ovallei, a rare and endange red geophyte of the Atacama Deserten_US
dc.typeArtículo de Revistaen_US
uct.catalogadormlmen_US
uct.comunidadRecursos Naturalesen_US
uct.disciplinaCiencias de las Plantasen_US
uct.indizacionSCOPUSen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Vargas, Jofre, Navarrete, Bravo, Jamett, Inostroza, Ibanez_Sexual_2018.pdf
Size:
522.55 KB
Format:
Adobe Portable Document Format
Description:
Artículo de revista
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
803 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Medio Ambiente