Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility

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datacite.alternateIdentifier.citationAnimal Reproduction Science, 209, 2019
datacite.alternateIdentifier.doi10.1016/j.anireprosci.2019.106133
datacite.alternateIdentifier.issn0378-4320
datacite.creatorLee-Estevez, Manuel
datacite.creatorHerrera, Lisandra
datacite.creatorDiaz, Rommy
datacite.creatorBeltran, Jorge
datacite.creatorFigueroa, Elias
datacite.creatorDumorne, Kelly
datacite.creatorUlloa-Rodriguez, Patricio
datacite.creatorShort, Stefania
datacite.creatorRisopatron, Jennie
datacite.creatorValdebenito, Ivan
datacite.creatorFarias, Jorge
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectCryopreservation
datacite.subjectSalmo salar
datacite.subjectMotility
datacite.subjectSpermatozoa
datacite.subjectPKA
datacite.subjectAMPK
datacite.titleEffects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:39Z
dc.date.available2025-10-06T14:21:39Z
dc.description.abstractSperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from energetic and cell signalling approaches. In this study, cAMP-dependent protein kinase (PKA) and AMP-activated kinase (AMPK) activities were quantified in fresh and cryopreserved spermatozoa of Atlantic salmon (Salmo salar); and the relation with motility was analysed. Results indicate there was a decrease in membrane integrity and motility in post-thawed spermatozoa compared to fresh samples, however, there was about 30% of cells with intact plasma membrane but incapable of motility. The PKA and AMPK activities were less after cryopreservation, indicating that loss of motility may be related to alteration of these key enzymes. Furthermore, PKA and AMPK activities were positively correlated with each other and with motility; and inhibition decreased motility, indicating there is a functional relationship between PKA and AMPK. The PKA inhibition also decreased AMPK activity, but results from protein-protein docking analyses indicated AMPK activation directly by PKA is unlikely, thus an indirect mechanism may exist. There have been no previous reports of these kinase actions in fish spermatozoa, making these findings worthy of assessment when there are future studies being planned, and may serve as base knowledge for optimization of cryopreservation procedures and development of biotechnologies to improve reproduction efficiency in the aquaculture industry.
dc.description.ia_keywordmotility, ampk, protein, kinase, there, cryopreservation, spermatozoa
dc.identifier.issn1873-2232
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6723
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAnimal Reproduction Science
dc.subject.ia_odsODS 9: Industria, innovación e infraestructura
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationTitleAnimal Reproduction Science
oaire.citationVolume209
oaire.fundingReferenceCONICYT ANID FONDECYT 1180387 (Regular), 3160572 (Postdoctorado)
oaire.fundingReferenceUFRO Postdoctoral 2019
oaire.licenseConditionCopyright © Elsevier Inc, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus|PubMed
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