A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage
Date
Authors
FIGUEROA VILLALOBOS, ELIAS GUSTAVO
Beltran, Jorge F.
Zamorano, Mauricio J.
Belén, Lisandra Herrera
Risopatron, Jennie
Valdebenito, Iván
Norambuena, Juan Alejandro
Farias, Joaquín A.
Farias, Jorge G.
Beltran, Jorge F.
Zamorano, Mauricio J.
Belén, Lisandra Herrera
Risopatron, Jennie
Valdebenito, Iván
Norambuena, Juan Alejandro
Farias, Joaquín A.
Farias, Jorge G.
Authors
Date
Datos de publicación:
10.1016/j.aquaculture.2021.737211
Keywords
Calcium - Channel - Motility - Proteomics - Salmo Salar - Concentration (composition) - Detection Method - Ion - Membrane - Motility - Plasma - Protein - Proteomics - Salmonid - Sperm - Salmo Salar
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Abstract
Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 °C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation. © 2021 Elsevier B.V., All rights reserved.
Description
Keywords
Calcium , Channel , Motility , Proteomics , Salmo Salar , Concentration (composition) , Detection Method , Ion , Membrane , Motility , Plasma , Protein , Proteomics , Salmonid , Sperm , Salmo Salar
Citation
10.1016/j.aquaculture.2021.737211