Technologies used in the study of sperm function in cryopreserved fish spermatozoa

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datacite.alternateIdentifier.citationAquaculture Research, 47 (6), 1705-1691, 2016
datacite.alternateIdentifier.doi10.1111/are.12630
datacite.alternateIdentifier.issn1355-557X
datacite.creatorFigueroa, Elias
datacite.creatorValdebenito, Ivan
datacite.creatorFarias, Jorge G.
datacite.date2016
datacite.rightsRegistro bibliográfico
datacite.subjectCryopreservation
datacite.subjectcryodamage
datacite.subjectfish spermatozoa
datacite.subjectfarming industry
datacite.subjectaquaculture
datacite.titleTechnologies used in the study of sperm function in cryopreserved fish spermatozoa
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:42Z
dc.date.available2025-10-06T14:21:42Z
dc.description.abstractSperm cryopreservation has led to transcendental changes in the reproductive biotechnology of both mammals and fish, and is a basic tool for animal improvement. However, these protocols generate damage to cell structure and physiology, altering sperm function as a result of cryoinjuries during freezing and thawing. This review is a compilation of the techniques developed and standardised for assessing sperm function in cryopreserved fish semen. Recent studies have analysed sperm function objectively, applying cellular and molecular techniques to characterise cryodamage. The Computer Assisted Sperm Analysis system has facilitated the assessment of motility, while electron microscopy (SEM and TEM) and cryo-microscopy have made it possible to study sperm morphology and ultra-structure. The effects of cryodamage on nuclear DNA have also been analysed using various methods, including the comet Fluorescence in situ Hybridization test, TUNEL, Sperm Chromatin Structure Assay, specific DNA sequences using RT-PCR and specific genes by qPCR. The latter technique is used to study the mitochondrial genome (mtDNA), together with some candidate genes which are associated with bioenergy activity and sperm motility. Other parameters assessed are mitochondrial membrane potential and ATP content using high performance liquid chromatography, nuclear magnetic resonance spectrometry and cell respiration. All this information makes it possible to establish study and assessment criteria for cryopreserved fish spermatozoa. This work focuses on the use of technologies to study of quality of fish spermatozoa during cryopreservation.
dc.description.ia_keywordsperm, fish, function, structure, cryopreserved, spermatozoa, cryopreservation
dc.identifier.issn1365-2109
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6762
dc.language.isoen
dc.publisherWiley
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAquaculture Research
dc.subject.ia_odsODS 8: Trabajo decente y crecimiento económico
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2016
oaire.citationEndPage1705
oaire.citationIssue6
oaire.citationStartPage1691
oaire.citationTitleAquaculture Research
oaire.citationVolume47
oaire.fundingReferenceANID FONDEF D10I1064
oaire.fundingReferenceANID FONDECYT 1120705 (Regular)
oaire.licenseConditionCopyright © John Wiley & Sons Ltd , 2014
oaire.resourceTypeArtículo de revisión
oaire.resourceType.enReview
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionWOS
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