Role of the subcommissural organ in the pathogenesis of congenital hydrocephalus in the HTx rat

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datacite.alternateIdentifier.citationCell and Tissue Research, 352 (3), 725-707, 2013
datacite.alternateIdentifier.doi10.1007/s00441-013-1615-9
datacite.alternateIdentifier.issn0302-766X
datacite.creatorOrtloff, Alexander R.
datacite.creatorVio, Karin
datacite.creatorGuerra, Montserrat
datacite.creatorJaramillo, Katherine
datacite.creatorKaehne, Thilo
datacite.creatorJones, Hazel
datacite.creatorMcAllister, James P., II
datacite.creatorRodriguez, Esteban
datacite.date2013
datacite.rightsRegistro bibliográfico
datacite.subjectHydrocephalus
datacite.subjectSubcommissural organ
datacite.subjectReissner's fibre
datacite.subjectCerebrospinal fluid
datacite.subjectHTx rat
datacite.titleRole of the subcommissural organ in the pathogenesis of congenital hydrocephalus in the HTx rat
dc.contributor.authorORTLOFF TRAUTMANN, ALEXANDER
dc.description.abstractThe present investigation was designed to clarify the role of the subcommissural organ (SCO) in the pathogenesis of hydrocephalus occurring in the HTx rat. The brains of non-affected and hydrocephalic HTx rats from embryonic day 15 (E15) to postnatal day 10 (PN10) were processed for electron microscopy, lectin binding and immunocytochemistry by using a series of antibodies. Cerebrospinal fluid (CSF) samples of non-affected and hydrocephalic HTx rats were collected at PN1, PN7 and PN30 and analysed by one- and two-dimensional electrophoresis, immunoblotting and nanoLC-ESI-MS/MS. A distinct malformation of the SCO is present as early as E15. Since stenosis of the Sylvius aqueduct (SA) occurs at E18 and dilation of the lateral ventricles starts at E19, the malformation of the SCO clearly precedes the onset of hydrocephalus. In the affected rats, the cephalic and caudal thirds of the SCO showed high secretory activity with all methods used, whereas the middle third showed no signs of secretion. At E18, the middle non-secretory third of the SCO progressively fused with the ventral wall of SA, resulting in marked aqueduct stenosis and severe hydrocephalus. The abnormal development of the SCO resulted in the permanent absence of Reissner's fibre (RF) and led to changes in the protein composition of the CSF. Since the SCO is the source of a large mass of sialilated glycoproteins that form the RF and of those that remain CSF-soluble, we hypothesize that the absence of this large mass of negatively charged molecules from the SA domain results in SA stenosis and impairs the bulk flow of CSF through the aqueduct.
dc.description.ia_keywordhydrocephalus, affected, rats, stenosis, aqueduct, present, role
dc.identifier.issn1432-0878
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3866
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceCell and Tissue Research
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2013
oaire.citationEndPage725
oaire.citationIssue3
oaire.citationStartPage707
oaire.citationTitleCell and Tissue Research
oaire.citationVolume352
oaire.fundingReferenceANID FONDECYT 1070241, 1111018 (Regular)
oaire.fundingReferenceHydrocephalus Association 51002705
oaire.fundingReferenceUACh DID S-2006-72
oaire.fundingReferenceCONICYT
oaire.fundingReferenceNICHD (USA)
oaire.licenseConditionCopyright © Springer, 2013
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication798755f1-1bb9-449a-b2e2-d4bbe38ca016
relation.isAuthorOfPublication.latestForDiscovery798755f1-1bb9-449a-b2e2-d4bbe38ca016
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionEMBASE
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