Granulocyte-macrophage colony stimulating factor (GM-CSF) enhances cumulus cell expansion in bovine oocytes

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datacite.alternateIdentifier.citationReproductive Biology and Endocrinology, 11, 2013
datacite.alternateIdentifier.doi10.1186/1477-7827-11-55
datacite.creatorPeralta, Oscar A.
datacite.creatorBucher, Danai
datacite.creatorFernandez, Ana
datacite.creatorBerland, Marco
datacite.creatorStrobel, Pablo
datacite.creatorRamirez, Alfredo
datacite.creatorRatto, Marcelo H.
datacite.creatorConcha, Ilona
datacite.date2013
datacite.rightsAcceso abierto
datacite.subjectGM-CSF
datacite.subjectOocyte maturation
datacite.subjectEmbryo development
datacite.subjectGM-CSF receptors
datacite.titleGranulocyte-macrophage colony stimulating factor (GM-CSF) enhances cumulus cell expansion in bovine oocytes
dc.contributor.authorBERLAND OLEA, MARCO ANTONIO
dc.description.abstractBackground: The objectives of the study were to characterize the expression of the alpha- and beta-subunits of granulocyte-macrophage colony stimulating factor (GM-CSF) receptor in bovine cumulus cells and oocytes and to determine the effect of exogenous GM-CSF on cumulus cells expansion, oocyte maturation, IGF-2 transcript expression and subsequent competence for embryonic development. Methods: Cumulus-oocyte complexes (COC) were obtained by aspirating follicles 3- to 8-mm in diameter with an 18 G needle connected to a vacuum pump at -50 mmHg. Samples of cumulus cells and oocytes were used to detect GM-CSF receptor by immunofluorescence. A dose-response experiment was performed to estimate the effect of GM-CSF on cumulus cell expansion and nuclear/cytoplasmic maturation. Also, the effect of GM-CSF on IGF-2 expression was evaluated in oocytes and cumulus cells after in vitro maturation by Q-PCR. Finally, a batch of COC was randomly assigned to in vitro maturation media consisting of: 1) synthetic oviductal fluid (SOF, n = 212); 2) synthetic oviductal fluid supplemented with 100 ng/ml of GM-CSF (SOF + GM-CSF, n = 224) or 3) tissue culture medium (TCM 199, n = 216) and then subsequently in vitro fertilized and cultured for 9 days. Results: Immunoreactivity for both alpha and beta GM-CSF receptors was localized in the cytoplasm of both cumulus cells and oocytes. Oocytes in vitro matured either with 10 or 100 ng/ml of GM-CSF presented a higher (P < 0.05) cumulus cells expansion than that of the control group (0 ng/ml of GM-CSF). GM-CSF did not affect the proportion of oocytes in metaphase II, cortical granules dispersion and IGF-2 expression. COC exposed to 100 ng/ml of GM-CSF during maturation did not display significant differences in terms of embryo cleavage rate (50.4% vs. 57.5%), blastocyst development at day 7 (31.9% vs. 28.7%) and at day 9 (17.4% vs. 17.9%) compared to untreated control (SOF alone, P = 0.2). Conclusions: GM-CSF enhanced cumulus cell expansion of in vitro matured bovine COC. However, GM-CSF did not increase oocyte nuclear or cytoplasmic maturation rates, IGF-2 expression or subsequent embryonic development.
dc.description.ia_keywordcumulus, oocytes, cells, maturation, expression, expansion, vitro
dc.formatPDF
dc.identifier.issn1477-7827
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3351
dc.language.isoen
dc.publisherBmc
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceReproductive Biology and Endocrinology
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2013
oaire.citationTitleReproductive Biology and Endocrinology
oaire.citationVolume11
oaire.fundingReferenceUACh Dirección de Investigación S-2009-26
oaire.fundingReferenceUCT 2007 DGI-CDA-04
oaire.fundingReferenceANID FONDECYT 1110508 (Regular)
oaire.licenseConditionObra bajo licencia Creative Commons Atribución 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication3fb21919-abd0-41cd-bb97-b32be4969ad4
relation.isAuthorOfPublication.latestForDiscovery3fb21919-abd0-41cd-bb97-b32be4969ad4
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed Central (PMC)
uct.indizacionPubMed
uct.indizacionEMBASE
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