Protective effects of polyunsatutared fatty acids supplementation against testicular damage induced by intermittent hypobaric hypoxia in rats

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datacite.alternateIdentifier.citationJournal of Biomedical Science, 22, 2015
datacite.alternateIdentifier.doi10.1186/s12929-015-0112-8
datacite.alternateIdentifier.issn1021-7770
datacite.creatorCastillo, Rodrigo L.
datacite.creatorZepeda, Andrea B.
datacite.creatorShort, Stefania E.
datacite.creatorFigueroa, Elias
datacite.creatorBustos-Obregon, Eduardo
datacite.creatorFarias, Jorge G.
datacite.date2015
datacite.rightsAcceso abierto
datacite.subjectIntermittent hypoxia
datacite.subjectTestis
datacite.subjectOxidative stress
datacite.subjectPUFA
datacite.subjectSIRTUIN 1
datacite.titleProtective effects of polyunsatutared fatty acids supplementation against testicular damage induced by intermittent hypobaric hypoxia in rats
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.date.accessioned2025-10-06T14:21:57Z
dc.date.available2025-10-06T14:21:57Z
dc.description.abstractBackground: Intermittent hypobaric hypoxia (IHH) induces changes in the redox status and structure in rat testis. These effects may be present in people at high altitudes, such as athletes and miners. Polyunsaturated fatty acids (PUFA) can be effective in counteracting these oxidative modifications due to their antioxidants properties. The aim of the work was to test whether PUFA supplementation attenuates oxidative damage in testis by reinforcing the antioxidant defense system. The animals were divided into four groups (7 rats per group): normobaric normoxia (similar to 750 tor; pO2 156 mmHg; Nx); Nx + PUFA, supplemented with PUFA (DHA: EPA = 3: 1; 0.3 g kg(-1) of body weight per day); hypoxic hypoxia (similar to 428 tor; pO2 90 mmHg; Hx) and, Hx + PUFA. The hypoxic groups were exposed in 4 cycles to 96 h of HH followed by 96 h of normobaric normoxia for 32 days. Total antioxidant capacity (FRAP) and lipid peroxidation (malondialdehyde, MDA) in plasma and reduced (GSH)/oxidized glutathione (GSSG) ratio, tissue lipid peroxidation (TBARS) and antioxidant enzymes activity were assessed at the end of the study in testis. Also, SIRTUIN 1 and HIF-1 protein expression in testis were determined. Results: IHH increased lipid peroxidation in plasma and HIF-1 protein levels in testis. In addition, IHH reduced FRAP levels in plasma, antioxidant enzymes activities and SIRTUIN 1 protein levels in testis. PUFA supplementation attenuated these effects, inducing the increases in FRAP, in the antioxidant enzymes activity and HIF-1 levels. Conclusions: These results suggest that the IHH model induces a prooxidant status in plasma and testis. The molecular protective effect of PUFA may involve the induction of an antioxidant mechanism.
dc.description.ia_keywordtestis, pufa, antioxidant, these, were, plasma, levels
dc.formatPDF
dc.identifier.issn1423-0127
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6875
dc.language.isoen
dc.publisherBiomed Central Ltd
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceJournal of Biomedical Science
dc.subject.ia_odsODS 8: Trabajo decente y crecimiento económico
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2015
oaire.citationTitleJournal of Biomedical Science
oaire.citationVolume22
oaire.fundingReferenceCONICYT ANID FONDECYT 11110426 (Regular)
oaire.fundingReferenceDIUFRO DI13-2014
oaire.licenseConditionObra bajo licencia Creative Commons Atribución 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionMEDLINE
uct.indizacionEMBASE
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