Genotoxic and oxidative stress potential of nanosized and bulk zinc oxide particles in Drosophila melanogaster

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datacite.alternateIdentifier.citationToxicology and industrial Health, 32 (12), 2001-1987, 2016
datacite.alternateIdentifier.doi10.1177/0748233715599472
datacite.alternateIdentifier.issn0748-2337
datacite.creatorCarmona, Erico R.
datacite.creatorInostroza-Blancheteau, Claudio
datacite.creatorRubio, Laura
datacite.creatorMarcos, Ricard
datacite.date2016
datacite.rightsRegistro bibliográfico
datacite.subjectComet assay
datacite.subjectDNA damage
datacite.subjecthaemocytes
datacite.subjectmalondialdehyde
datacite.subjectwing-spot test
datacite.subjectZnO
datacite.titleGenotoxic and oxidative stress potential of nanosized and bulk zinc oxide particles in Drosophila melanogaster
dc.contributor.authorINOSTROZA BLANCHETEAU, CLAUDIO ANDRES
dc.description.abstractZinc oxide nanoparticles (ZnONP) are manufactured on a large scale and can be found in a variety of consumer products, such as sunscreens, lotions, paints and food additives. Few studies have been carried out on its genotoxic potential and related mechanisms in whole organisms. In the present study, the in vivo genotoxic activity of ZnONP and its bulk form was assayed using the wing-spot test and comet assay in Drosophila melanogaster. Additionally, a lipid peroxidation analysis using the thiobarbituric acid assay was also performed. Results obtained with the wing-spot test showed a lack of genotoxic activity of both ZnO forms. However, when both particle sizes were tested in the comet assay using larvae haemocytes, a significant increase in DNA damage was observed for ZnONP treatments but only at the higher dose applied. In addition, the lipid per oxidation assay showed significant malondialdehyde (MDA) induction for both ZnO forms, but the induction of MDA for ZnONP was higher for the ZnO bulk, suggesting that the observed DNA strand breaks could be induced by mediated oxidative stress. The overall data suggest that the potential genotoxicity of ZnONP in Drosophila can be considered weak according to the lack of mutagenic and recombinogenic effects and the induction of primary DNA damage only at high toxic doses of ZnONP. This study is the first assessing the genotoxic and oxidative stress potential of nano and bulk ZnO particles in Drosophila.
dc.description.ia_keywordznonp, genotoxic, potential, bulk, assay, drosophila, both
dc.identifier.issn1477-0393
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3220
dc.language.isoen
dc.publisherSAGE Publishing
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceToxicology and industrial Health
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2016
oaire.citationEndPage2001
oaire.citationIssue12
oaire.citationStartPage1987
oaire.citationTitleToxicology and industrial Health
oaire.citationVolume32
oaire.fundingReferenceCONICYT ANID FONDECYT 11110181 (Regular)
oaire.fundingReferenceUCT DGIP CD2010-01
oaire.fundingReferenceMECESUP UCT 0804
oaire.licenseConditionCopyright © SAGE Publications, 2016
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication25287ee1-cae8-401d-b42c-ee3f8fc05d2a
relation.isAuthorOfPublication.latestForDiscovery25287ee1-cae8-401d-b42c-ee3f8fc05d2a
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionEMBASE
uct.indizacionTOXLINE
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