The effects of intraperitoneal administration of Francisella noatunensis subsp. noatunensis on hepatic intermediary metabolism and indicators of stress in Patagonian blennie Eleginops maclovinus

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datacite.alternateIdentifier.citationComparative Biochemistry and Physiology -Part B: Biochemistry and Molecular Biology, 230, 56-48, 2019
datacite.alternateIdentifier.doi10.1016/j.cbpb.2019.01.013
datacite.alternateIdentifier.issn1096-4959
datacite.creatorOyarzún-Salazar, Ricardo
datacite.creatorVargas-Lagos, Carolina
datacite.creatorMartínez, Danixa Pamela
datacite.creatorMuñoz, José L.P.
datacite.creatorDantagnan, Patricio P.
datacite.creatorVargas-Chacoff, Luis
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectEleginops Maclovinus
datacite.subjectFrancisella Noatunensis
datacite.subjectIntermediary Metabolism
datacite.subjectIntraperitoneal Administration
datacite.subjectStress
datacite.subjectAlanine Aminotransferase
datacite.subjectGlucose
datacite.subjectGlucose 6 Phosphate Dehydrogenase
datacite.subjectGlutamate Dehydrogenase
datacite.subjectHydrocortisone
datacite.subjectLactate Dehydrogenase
datacite.subjectLactate Dehydrogenase A
datacite.subjectLactic Acid
datacite.subjectAlanine Aminotransferase
datacite.subjectGlucose
datacite.subjectGlucose 6 Phosphate Dehydrogenase
datacite.subjectGlutamate Dehydrogenase
datacite.subjectHydrocortisone
datacite.subjectLactate Dehydrogenase
datacite.subjectLactic Acid
datacite.subjectLiver Enzyme
datacite.subjectTriacylglycerol
datacite.subjectAnimal Experiment
datacite.subjectAnimal Model
datacite.subjectAnimal Tissue
datacite.subjectArticle
datacite.subjectCellular Stress Response
datacite.subjectControlled Study
datacite.subjectEleginops Maclovinus
datacite.subjectEnergy Transfer
datacite.subjectEnzyme Activity
datacite.subjectFish
datacite.subjectFish Disease
datacite.subjectFrancisella
datacite.subjectFrancisella Noatunensis Subsp Noatunensis
datacite.subjectFrancisellosis
datacite.subjectGlucose Blood Level
datacite.subjectLactate Blood Level
datacite.subjectLiver Metabolism
datacite.subjectMale
datacite.subjectMetabolite
datacite.subjectNonhuman
datacite.subjectPriority Journal
datacite.subjectStress
datacite.subjectTriacylglycerol Blood Level
datacite.subjectAnimal
datacite.subjectGram Negative Infection
datacite.subjectIntraperitoneal Drug Administration
datacite.subjectLiver
datacite.subjectMetabolism
datacite.subjectMicrobiology
datacite.subjectPathogenicity
datacite.subjectPathology
datacite.subjectPerciformes
datacite.subjectPhysiological Stress
datacite.subjectAnimals
datacite.subjectFish Diseases
datacite.subjectGram-negative Bacterial Infections
datacite.subjectInjections, Intraperitoneal
datacite.subjectLiver
datacite.subjectStress, Physiological
datacite.titleThe effects of intraperitoneal administration of Francisella noatunensis subsp. noatunensis on hepatic intermediary metabolism and indicators of stress in Patagonian blennie Eleginops maclovinus
dc.contributor.authorDANTAGNAN DANTAGNAN, HERMAN PATRICIO
dc.description.abstractFrancisellosis is a disease produced by Francisella spp. which affects farmed fish. Eleginops maclovinus specimens can be caught close to salmon farming centers, feeding on un-consumed pellet, making the transmission of pathogens such as Francisella noatunensis possible. The aim of this study was to evaluate the effect of F. noatunensis on liver intermediary metabolism in E. maclovinus. 144 fish were injected intraperitoneally with F. noatunensis at a low dose LD (1 × 101 cells/?L), medium dose MD (1 × 105 cells/?L), high dose HD (1 × 1010 cells/?L), or with culture medium C (control), and sampled at 1, 3, 7, 14, 21 and 28 days post injection (dpi). No mortality was recorded during the experimental period, but there was a marked metabolic response in fish injected with high doses. Metabolites in plasma were lowest in the high bacterial dose. Cortisol levels were highest at day 7 in the high dose and then decreased from day 14 until the end of the study. Liver enzymes showed a similar pattern to plasma metabolites, with decreased enzymatic activity, mostly with the high bacteria dose. PK was the exception, with increased enzymatic activity in a dose-dependent manner over time. Liver metabolites were highly variable, except in the high bacterial dose where variability and total levels decreased significantly. Our results show that fish infection with F. noatunensis induces a clear stress response, especially with at the highest dose, shifting intermediary metabolism towards mobilization of energy and suggesting that E. maclovinus detects experimental infection of F. noatunensis as a stressor, which it is dependent on the bacterial dose. © 2020 Elsevier B.V., All rights reserved.
dc.description.ia_keyworddose, noatunensis, high, fish, maclovinus, were, francisella
dc.identifier.issn1879-1107
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3510
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceComparative Biochemistry and Physiology -Part B: Biochemistry and Molecular Biology
dc.subject.ia_odsODS 3: Salud y bienestar
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationEndPage56
oaire.citationStartPage48
oaire.citationTitleComparative Biochemistry and Physiology -Part B: Biochemistry and Molecular Biology
oaire.citationVolume230
oaire.fundingReferenceANID FONDAP 15150003 (IDEAL)
oaire.fundingReferenceANID FONDECYT 1160877 (Regular)
oaire.fundingReferenceCONICYT PFCHA Doctorado Nacional 2016-21160204
oaire.fundingReferenceUniversidad Austral de Chile (VIDCA)
oaire.licenseConditionCopyright © Elsevier Inc, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9ed02e88-c517-44ff-b002-91dfa5b40b99
relation.isAuthorOfPublication.latestForDiscovery9ed02e88-c517-44ff-b002-91dfa5b40b99
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionEMBASE
uct.indizacionCAB Abstracts
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