Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm

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datacite.alternateIdentifier.citationAquaculture, 481, 63-58, 2017
datacite.alternateIdentifier.doi10.1016/j.aquaculture.2017.08.022
datacite.alternateIdentifier.issn0044-8486
datacite.creatorContreras, P.
datacite.creatorUlloa, P.
datacite.creatorMerino, O.
datacite.creatorValdebenito, I.
datacite.creatorFigueroa, E.
datacite.creatorFarias, J.
datacite.creatorRisopatron, J.
datacite.date2017
datacite.rightsRegistro bibliográfico
datacite.subjectSperm storage
datacite.subjectSperm function
datacite.subjectEleginops maclovinus
datacite.titleEffect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:42Z
dc.date.available2025-10-06T14:21:42Z
dc.description.abstractShort-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameters in Patagonian blenny. Samples of undiluted (C: control) and diluted semen 1: 1 v/v (DS1) and 1: 3 v/v (DS2) in Storfish (R) were stored at 4 degrees C for 7 days. Motility, viability, mitochondrial membrane potential (Delta Psi Mit), DNA fragmentation and ATP content were assessed at 0, 3 and 7 days. The results demonstrated that sperm function parameters were affected significantly by the time of storage, and that dilution allows better preservation of sperm motility. On the third day of storage, spermatozoa showed a motility> 56% in DS1 and DS2, nevertheless, in C it significantly decreased as well as its ATP content (P < 0.05), whereas viability was maintained> 70% and Delta Psi Mit>45%. However, a significant decline in the percentage of sperm function parameters (P < 0.05) was observed at 7 days of storage for all samples of undiluted and diluted semen. DNA fragmentation showed no significant increase in all groups, demonstrating<5% at day 7. In vitro storage conditions maintain better sperm motility in diluted than undiluted semen, preserving the sperm function in all groups assessed during 3 days. In conclusion, the results provide new data on Patagonian blenny sperm quality with respect to in vitro short-term storage evaluated by flow cytometry, this information must be considered for implementing in aquaculture reproductive strategies for the conservation of this species.
dc.description.ia_keywordsperm, storage, semen, function, patagonian, blenny, days
dc.identifier.issn1873-5622
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6754
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAquaculture
dc.subject.ia_odsODS 14: Vida submarina
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nEcología
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2017
oaire.citationEndPage63
oaire.citationStartPage58
oaire.citationTitleAquaculture
oaire.citationVolume481
oaire.fundingReferenceCONICYT ANID FONDECYT 1151315 (Regular)
oaire.fundingReferenceCONICYT ANID FONDEF D06I1020
oaire.licenseConditionCopyright © Elsevier B.V, 2017
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionWOS
uct.indizacionDOAJ
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