?-NGF stimulates steroidogenic enzyme and VEGFA gene expression, and progesterone secretion via ERK 1/2 pathway in primary culture of llama Granulosa cells

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datacite.alternateIdentifier.citationFrontiers in Veterinary Science, 7, 2020
datacite.alternateIdentifier.doi10.3389/fvets.2020.586265
datacite.alternateIdentifier.issn2297-1769
datacite.creatorValderrama, Ximena P.
datacite.creatorUlloa-Leal, Cesar
datacite.creatorSilva, Mauricio Erciario
datacite.creatorVargas, José Goicochea
datacite.creatorApichela, Silvana A.
datacite.creatorArgañaraz, M. E.
datacite.creatorSari, Luciana M.
datacite.creatorPaiva, Luis
datacite.creatorRatto, Vicente Francisco
datacite.creatorRatto, M. Hector
datacite.date2020
datacite.rightsAcceso abierto
datacite.subjectCorpus Luteum
datacite.subjectCyp11a1
datacite.subjectCyp19a1
datacite.subjectFollicle
datacite.subjectHsd3b1
datacite.subjectMapk
datacite.subjectStar
datacite.subjectU0126
datacite.subject1,4 Diamino 1,4 Bis(2 Aminophenylthio) 2,3 Dicyanobutadiene
datacite.subject3(or 17)beta Hydroxysteroid Dehydrogenase
datacite.subjectAromatase
datacite.subjectCholesterol Monooxygenase (side Chain Cleaving)
datacite.subjectLuteinizing Hormone
datacite.subjectMitogen Activated Protein Kinase 1
datacite.subjectMitogen Activated Protein Kinase 3
datacite.subjectProgesterone
datacite.subjectVasculotropin A
datacite.subject1,4 Diamino 1,4 Bis(2 Aminophenylthio) 2,3 Dicyanobutadiene
datacite.subject3(or 17)beta Hydroxysteroid Dehydrogenase
datacite.subjectAromatase
datacite.subjectCholesterol Monooxygenase (side Chain Cleaving)
datacite.subjectComplementary Dna
datacite.subjectLuteinizing Hormone
datacite.subjectMessenger Rna
datacite.subjectMitogen Activated Protein Kinase 1
datacite.subjectMitogen Activated Protein Kinase 3
datacite.subjectNerve Growth Factor Beta Subunit
datacite.subjectNerve Growth Factor Receptor
datacite.subjectProgesterone
datacite.subjectProtein Tyrosine Kinase A
datacite.subjectVasculotropin A
datacite.subjectAdult
datacite.subjectAngiogenesis
datacite.subjectAnimal Cell
datacite.subjectAnimal Tissue
datacite.subjectArticle
datacite.subjectCell Culture
datacite.subjectControlled Study
datacite.subjectCorpus Luteum
datacite.subjectDown Regulation
datacite.subjectEnzyme Activation
datacite.subjectEnzyme Phosphorylation
datacite.subjectFemale
datacite.subjectFollicular Aspiration
datacite.subjectGene Expression
datacite.subjectGranulosa Cell
datacite.subjectIn Vitro Study
datacite.subjectLlama
datacite.subjectLuteinizing Hormone Blood Level
datacite.subjectMale
datacite.subjectMapk Signaling
datacite.subjectMrna Expression Level
datacite.subjectNonhuman
datacite.subjectOvary Follicle
datacite.subjectOvary Tissue
datacite.subjectOvulation
datacite.subjectPrimary Culture
datacite.subjectProgesterone Release
datacite.subjectProgesterone Synthesis
datacite.subjectRadioimmunoassay
datacite.subjectReal Time Polymerase Chain Reaction
datacite.subjectRna Isolation
datacite.subjectRna Transcription
datacite.subjectSemen Analysis
datacite.subjectSteroidogenesis
datacite.subjectTransvaginal Echography
datacite.subjectWestern Blotting
datacite.title?-NGF stimulates steroidogenic enzyme and VEGFA gene expression, and progesterone secretion via ERK 1/2 pathway in primary culture of llama Granulosa cells
dc.contributor.authorSILVA JIMENEZ, MAURICIO
dc.description.abstractThe beta-nerve growth factor (?-NGF) from llama seminal plasma exerts ovulatory and luteotrophic effects following intramuscular or intrauterine infusion in llamas and alpacas. In this study, we investigate the in vitro effect of llama ?-NGF on the expression of genes involved in angiogenesis and progesterone synthesis as well as progesterone release in preovulatory llama granulosa cells; we also determine whether these changes are mediated via the ERK1/2 signaling pathway. From adult female llamas, we collected granulosa cells from preovulatory follicles by transvaginal ultrasound-guided follicle aspiration; these cells were pooled and incubated. After 80% confluence, the cultured granulosa cells were treated with ?-NGF,?-NGF plus the MAPK inhibitor U0126, or luteinizing hormone, and the abundance of angiogenic and steroidogenic enzyme mRNA transcripts were quantified after 10 and 20 h by RT-qPCR. We also quantified the progesterone concentration in the media after 48 h by radioimmunoassay. We found that application of ?-NGF increases the abundance of mRNA transcripts of the vascular endothelial growth factor (VEGFA) and the steroidogenic enzymes cytochrome P450 side-chain cleavage (P450scc/CYP11A1), steroidogenic acute regulatory protein (STAR), and 3?-hydroxysteroid dehydrogenase (HSD3B1) at 10 and 20 h of treatment. Application of the MAPK inhibitor U0126 resulted in downregulation of the genes encoding these enzymes. ?-NGF also enhanced progesterone synthesis, which was prevented by the prior application of the MAPK inhibitor U0126. Finally, western blot analysis confirmed that ?-NGF activates the ERK1/2 signaling pathway. In conclusion, our results indicate that ?-NGF exerts direct luteotropic effects on llama ovarian tissue via the ERK 1/2 pathway. © 2021 Elsevier B.V., All rights reserved.
dc.description.ia_keywordllama, progesterone, cells, granulosa, pathway, steroidogenic, also
dc.formatPDF
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3920
dc.language.isoen
dc.publisherFrontiers Media
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceFrontiers in Veterinary Science
dc.subject.ia_odsODS 5: Igualdad de género
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2020
oaire.citationTitleFrontiers in Veterinary Science
oaire.citationVolume7
oaire.fundingReferenceANID FONDECYT 3200439 (Postdoctorado), 1160934 (Regular)
oaire.licenseConditionObra bajo licencia Creative Commons Atribución 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication5a5fa19b-bce1-4387-8139-279b35112838
relation.isAuthorOfPublication.latestForDiscovery5a5fa19b-bce1-4387-8139-279b35112838
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionDOAJ
uct.indizacionPubMed
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