Intra-amoebic localization of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

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datacite.alternateIdentifier.citationArchives of Microbiology, 201 (10), 1452-1447, 2019
datacite.alternateIdentifier.doi10.1007/s00203-019-01699-9
datacite.alternateIdentifier.issn1432-072X
datacite.creatorMedina, Gustavo A.
datacite.creatorLeyán, Pilar I.
datacite.creatorda Silva, Claudio Vieira
datacite.creatorFlores-Martin, Sandra N.
datacite.creatorManosalva, Carolina
datacite.creatorFernández, Heriberto
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectAcanthamoeba
datacite.subjectArcobacter
datacite.subjectConfocal Microscopy
datacite.subjectEndosymbiosis
datacite.subjectAcanthamoeba Castellanii
datacite.subjectActin Polymerization
datacite.subjectArcobacter Butzleri
datacite.subjectArticle
datacite.subjectBacterial Metabolism
datacite.subjectBacterial Survival
datacite.subjectBacterium Culture
datacite.subjectCell Vacuole
datacite.subjectCitric Acid Cycle
datacite.subjectCoculture
datacite.subjectConfocal Microscopy
datacite.subjectEndoplasmic Reticulum
datacite.subjectEndosymbiosis
datacite.subjectEnergy Yield
datacite.subjectFlow Cytometry
datacite.subjectHost Cell
datacite.subjectIn Vitro Study
datacite.subjectLegionella Pneumophila
datacite.subjectMacrophage
datacite.subjectNonhuman
datacite.subjectPriority Journal
datacite.subjectArcobacter
datacite.subjectMicrobiology
datacite.subjectPhysiology
datacite.subjectSymbiosis
datacite.subjectSymbiosis
datacite.subjectVacuoles
datacite.titleIntra-amoebic localization of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii
dc.date.accessioned2025-10-06T14:21:43Z
dc.date.available2025-10-06T14:21:43Z
dc.description.abstractAcanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing their association with endoplasmic reticulum vesicles and mitochondria. Through confocal microscopy, we observed that during the early stages of endosymbiosis, there is not colocalization between amoebic vacuoles containing A. butzleri and mitochondria or ER vesicles of A. castellanii. Considering that energy production of this bacterium occurs via metabolism of amino acids or the tricarboxylic acid cycle, these results contribute to explain the absence of bacterial replication, since A. butzleri would not have access to the nutrients found in endoplasmic reticulum vesicles and mitochondria. In addition, we observe that A. butzleri induces significantly the actin polymerization of A. castellanii during the early stages of endosymbiosis. © 2019 Elsevier B.V., All rights reserved.
dc.description.ia_keywordbutzleri, castellanii, vesicles, mitochondria, acanthamoeba, found, arcobacter
dc.identifier.issn0302-8933
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6765
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceArchives of Microbiology
dc.subject.ia_odsODS 7: Energía asequible y no contaminante
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationEndPage1452
oaire.citationIssue10
oaire.citationStartPage1447
oaire.citationTitleArchives of Microbiology
oaire.citationVolume201
oaire.fundingReferenceANID FONDECYT 1110202 (Regular)
oaire.fundingReferenceCONICYT AT 24121322
oaire.fundingReferenceUCT FEQUIP2018-PL-06
oaire.licenseConditionCopyright © Springer-Verlag GmbH, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
uct.catalogadorjvu
uct.comunidadCiencias de la Saluden_US
uct.departamentoDepartamento de Procesos Diagnósticos y Evaluación
uct.facultadFacultad de Ciencias de la Salud
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionScimago
uct.indizacionPubMed
uct.indizacionEMBASE
uct.indizacionLatindex
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