Enhancing Laccase and Manganese Peroxidase Activity in White-Rot Fungi: The Role of Copper, Manganese, and Lignocellulosic Substrates
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Authors
PINTO IBIETA, FERNANDA EMILIA
Benavides, Viviana
Ciudad, Gustavo A.
Pinto-Ibieta, Fernanda
Robledo-Mahón, Tatiana
Rubilar, Olga
Serrano, Antonio
Benavides, Viviana
Ciudad, Gustavo A.
Pinto-Ibieta, Fernanda
Robledo-Mahón, Tatiana
Rubilar, Olga
Serrano, Antonio
Authors
Date
Datos de publicación:
10.3390/agronomy14112562
Keywords
Laccase - Ligninolytic Enzymes - Lignocellulosic Substrates - Manganese Peroxidase - White-rot Fungi - Hongos de podredumbre blanca - Enzimas ligninolíticas - Lacasa - Peroxidasa de manganeso - Sustratos lignocelulósicos
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Abstract
White-rot fungi (WRF) are increasingly recognized for their biotechnological potential due to the wide range of applications of ligninolytic enzymes. The addition of different metals involved in the functioning of ligninolytic enzymes, mainly copper and manganese, has been widely studied to maximize the enzymatic activities of the WRF. This review aims to provide information on the effect of metal-fungi interaction mechanisms that justify the effects of enzymatic activity. The addition of copper is associated with increased laccase activity, with reported improvements in the laccase activity compared to controls without metal addition of up to 100% at doses between 0.5 1 mM. The addition of manganese resulted in an improvement in manganese peroxidase activity with respect to the control at the wide range of 1 18.2 mM. Furthermore, enzymatic activity was generally favored by using substrates with lignocellulosic fibers with respect to synthetic culture medium. Quantifying the concentration of metals in the substrate is required to monitor bioavailable metals for fungi in these assays accurately, making an external contribution less necessary. © 2024 Elsevier B.V., All rights reserved.
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Keywords
Laccase , Ligninolytic Enzymes , Lignocellulosic Substrates , Manganese Peroxidase , White-rot Fungi , Hongos de podredumbre blanca , Enzimas ligninolíticas , Lacasa , Peroxidasa de manganeso , Sustratos lignocelulósicos
Citation
10.3390/agronomy14112562