Effects of diet on sperm functionality and cryopreservation tolerance in Atlantic salmon (Salmo salar)

Simple item page
datacite.alternateIdentifier.citationTheriogenology, 247, 2025
datacite.alternateIdentifier.doi10.1016/j.theriogenology.2025.117565
datacite.alternateIdentifier.issn0093-691X
datacite.creatorVillalobos, Elías Figueroa
datacite.creatorMerino, Osvaldo
datacite.creatorAmorim Pereira, Wellison
datacite.creatorPérez-Atehortúa, Maritza
datacite.creatorAvila, Sebastián
datacite.creatorPinheiro, Ricardo
datacite.creatorHernández Arias, Adrián J.
datacite.creatorRomero, Jaime
datacite.creatorValdebenito Isler, Iván
datacite.creatorVillasante, Alejandro
datacite.date2025
datacite.rightsRegistro bibliográfico
datacite.subjectCryopreservation
datacite.subjectDietary Effect
datacite.subjectDna Fragmentation
datacite.subjectOxidative Stress
datacite.subjectSperm Function
datacite.subjectAnimal
datacite.subjectAnimal Food
datacite.subjectCryopreservation
datacite.subjectDiet
datacite.subjectDna Fragmentation
datacite.subjectMale
datacite.subjectOxidative Stress
datacite.subjectPhysiology
datacite.subjectSalmo Salar
datacite.subjectSemen Analysis
datacite.subjectSperm Preservation
datacite.subjectSpermatozoon
datacite.subjectSpermatozoon Motility
datacite.subjectVeterinary Medicine
datacite.subjectAnimal Feed
datacite.subjectAnimals
datacite.subjectCryopreservation
datacite.subjectDiet
datacite.subjectDna Fragmentation
datacite.subjectMale
datacite.subjectOxidative Stress
datacite.subjectSemen Analysis
datacite.subjectSemen Preservation
datacite.subjectSperm Motility
datacite.subjectSpermatozoa
datacite.titleEffects of diet on sperm functionality and cryopreservation tolerance in Atlantic salmon (Salmo salar)
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorFARIAS VEGA, CRISTIAN ALEX
dc.contributor.authorHERNANDEZ ARIAS, ADRIAN JESUS
dc.date.accessioned2025-10-06T14:22:10Z
dc.date.available2025-10-06T14:22:10Z
dc.description.abstractThis study evaluates the impact of dietary composition on the functionality and cryopreservation tolerance of intratesticular spermatozoa in Atlantic salmon (Salmo salar). A total of 40 males were divided into four dietary treatment groups: Diet I, based on marine-derived lipids and proteins; Diet II, replacing 65 % of marine proteins and 51 % of lipids with plant and terrestrial animal sources; and two commercial reference diets (III and IV) to contrast with the experimental diets. Over a six-month period, dietary treatments influenced critical sperm quality parameters such as motility, membrane integrity, oxidative stress markers, and cryopreservation outcomes as assessed in both fresh and cryopreserved samples using biomarkers of cellular function, mitochondrial membrane potential, and DNA integrity. Sperm in Diet 1 had the highest motility (80.6 ± 5.7 % fresh; 68.4 ± 8.7 % cryopreserved) and showed reduced oxidative damage, with lower levels of lipid peroxidation (3.8 ± 0.9 nmol MDA/mL) and 8-OHdG concentrations (0.7 ± 0.4 ng/mL). Conversely, sperm from Diets III and IV exhibited higher oxidative stress and DNA fragmentation, which were associated with reduced post-thaw viability. The results underscore the critical role of dietary polyunsaturated fatty acids (PUFAs), such as DHA and EPA, in maintaining sperm membrane fluidity, motility, and resistance to cryopreservation. Diet II exhibited intermediate performance, indicating that partial substitution of marine ingredients can be viable if lipid composition is carefully balanced. These findings emphasize the importance of dietary formulation in aquaculture for optimizing sperm quality and cryopreservation tolerance, contributing to sustainable reproduction strategies for Atlantic salmon and other high-value species. © 2025 Elsevier B.V., All rights reserved.
dc.description.ia_keywordsperm, dietary, cryopreservation, diet, tolerance, atlantic, salmon
dc.identifier.issn1879-3231
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6960
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceTheriogenology
dc.subject.ia_odsODS 14: Vida submarina
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2025
oaire.citationTitleTheriogenology
oaire.citationVolume247
oaire.fundingReferenceANID FONDECYT 11230690 (Iniciación), 1211246 (Regular)
oaire.fundingReferenceFAPESP Brasil 2018/25511-1, 2021/01570-1, 2022/07543-9
oaire.fundingReferenceUniversidad Católica de Temuco FEQUIP 2023FEQUIP-RB-02, 2024FEQUIP-NV-01
oaire.fundingReferenceHendrix Genetics Aquaculture S.A. (Chile)
oaire.licenseConditionCopyright © Elsevier Inc, 2025
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublication5f4b56d8-91cd-46a3-bc83-c2fda5280b97
relation.isAuthorOfPublicationd6879a45-cf2a-418d-8f5e-0773cf35f4dd
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionMEDLINE
uct.indizacionCAB Abstracts
uct.indizacionVeterinary Bulletin
Files
Collections
Producción Alimentaria