Effect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation

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datacite.alternateIdentifier.citationTheriogenology, 189, 10-1, 2022
datacite.alternateIdentifier.doi10.1016/j.theriogenology.2022.06.005
datacite.alternateIdentifier.issn0093-691X
datacite.creatorContreras, Maria José
datacite.creatorÁrias, María Elena
datacite.creatorSilva, Mauricio Erciario
datacite.creatorCabrera, Paulina
datacite.creatorFelmer, Ricardo N.
datacite.date2022
datacite.rightsRegistro bibliográfico
datacite.subjectCapacitation
datacite.subjectCholestanol
datacite.subjectCholesterol
datacite.subjectCryopreservation
datacite.subjectCyclodextrin
datacite.subjectStallion Sperm
datacite.subjectCholestanol
datacite.subjectCholesterol
datacite.subjectCyclodextrin
datacite.subjectCholestanol
datacite.subjectCholesterol
datacite.subjectCyclodextrins
datacite.subjectCholestanol
datacite.subjectCholesterol
datacite.subjectCyclodextrin
datacite.subjectAnimal
datacite.subjectBovine
datacite.subjectCryopreservation
datacite.subjectHorse
datacite.subjectMale
datacite.subjectPhysiology
datacite.subjectPlant Seed
datacite.subjectProcedures
datacite.subjectSperm Capacitation
datacite.subjectSperm Preservation
datacite.subjectSpermatozoon
datacite.subjectSpermatozoon Motility
datacite.subjectVeterinary Medicine
datacite.subjectAnimals
datacite.subjectCattle
datacite.subjectCholestanol
datacite.subjectCholesterol
datacite.subjectCryopreservation
datacite.subjectCyclodextrins
datacite.subjectHorses
datacite.subjectMale
datacite.subjectSeeds
datacite.subjectSemen Preservation
datacite.subjectSperm Capacitation
datacite.subjectSperm Motility
datacite.subjectSpermatozoa
datacite.titleEffect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation
dc.contributor.authorSILVA JIMENEZ, MAURICIO
dc.description.abstractCryopreservation of stallion semen is less efficient than other species such as bovine. This is mainly because of the greater susceptibility of stallion sperm to the freezing damage that generates oxidative stress and plasma membrane injury, resulting in DNA fragmentation and cell death. These data suggest the need to develop new strategies of sperm cryopreservation that can improve the efficiency of this technique in stallions by reducing or preventing membrane damage and cell death. The present study aimed to evaluate the effect of adding membrane stabilizers to the freezing medium and assess the quality and in vitro capacitation of stallion sperm after thawing. Semen samples from three stallions frozen with membrane stabilizers (cholesterol-loaded cyclodextrin and cholestanol-loaded cyclodextrin) were evaluated in two experiments: i) sperm quality and functional analysis after thawing, and ii) sperm quality and functional analysis after 4 h of post-thaw incubation in capacitating conditions. Plasma membrane integrity, mitochondrial membrane potential, membrane lipid disorder, intracellular Ca2+, tyrosine phosphorylation, acrosome reaction, DNA damage, sperm motility, and binding to the zona pellucida were assessed. The results showed that cholesterol-loaded cyclodextrin was the stabilizer that most efficiently reduced the membrane disruption and post-thaw cell damage. In addition, this stabilizer made it possible to obtain in vitro capacitated sperm showing higher plasma membrane integrity, mitochondrial membrane potential, sperm motility, binding to the zona pellucida and better response to in vitro capacitating conditions. © 2022 Elsevier B.V., All rights reserved.
dc.description.ia_keywordmembrane, sperm, stallion, damage, loaded, cyclodextrin, cryopreservation
dc.identifier.issn1879-3231
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4660
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceTheriogenology
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2022
oaire.citationEndPage10
oaire.citationStartPage1
oaire.citationTitleTheriogenology
oaire.citationVolume189
oaire.fundingReferenceANID FONDECYT 1160467
oaire.fundingReferenceANID DOCTORADO NACIONAL 21181068
oaire.fundingReferenceFRIGORÍFICO TEMUCO (aporte en especie)
oaire.licenseConditionCopyright © Elsevier Inc, 2022
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication5a5fa19b-bce1-4387-8139-279b35112838
relation.isAuthorOfPublication.latestForDiscovery5a5fa19b-bce1-4387-8139-279b35112838
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionMEDLINE
uct.indizacionCAB Abstracts
uct.indizacionVeterinary Bulletin
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