Endophytic seed-associated microbial diversity and its impact on seedling growth of the Andean tree Nothofagus obliqua (Mirb.) Oerst

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datacite.alternateIdentifier.citationPlant Growth Regulation, 103 (2), 336-321, 2024
datacite.alternateIdentifier.doi10.1007/s10725-023-01113-x
datacite.alternateIdentifier.issn0167-6903
datacite.creatorAlvarado, Roxana
datacite.creatorSagredo-Saez, Cristiane
datacite.creatorFuentes-Quiroz, Alejandra
datacite.creatorVillanueva-Guerrero, Angela
datacite.creatorMujica, Maria Isabel
datacite.creatorAhumada, Rodrigo
datacite.creatorAlmonacid, Leonardo
datacite.creatorJorquera-Fontena, Emilio
datacite.creatorde Oliveira Costa, Paulo Henrique
datacite.creatorda Silva Valadares, Rafael Borges
datacite.date2024
datacite.rightsRegistro bibliográfico
datacite.subjectEndophytes
datacite.subjectMetabarcoding
datacite.subjectNative Forest Tree
datacite.subjectPlant-growth Promotion
datacite.subjectRahnella Aquatilis
datacite.subjectSymbiosis
datacite.titleEndophytic seed-associated microbial diversity and its impact on seedling growth of the Andean tree Nothofagus obliqua (Mirb.) Oerst
dc.contributor.authorJORQUERA FONTENA, EMILIO JOSE
dc.description.abstractSymbiotic microorganisms are essential for promoting plant growth and establishment from the early stages of plant development. However, the diversity of seed-associated endophytes in native Andean trees and their role in growth promotion and seedling establishment have scarcely been studied. This study aimed to characterize the microbial diversity associated with seeds of Nothofagus obliqua (Mirb.) Oerst. Viable seeds were collected from healthy young trees in a section of the Nahuelbuta Mountains, south-central Chile. Then, they were processed to characterize total microbial diversity using a 16S rRNA gene and an internal transcribed spacer (ITS) region metabarcoding approach. The diversity of culturable bacteria was determined and tested for plant growth-promoting effects. Effects on seed germination, seedling development, and plantlet establishment were evaluated by in vivo inoculations. Seed-associated microbial diversity was dominated by Ascomycota and Proteobacteria, with Diaphorte and Pantoea being the most abundant genera. Five different strains of culturable bacteria were identified, with Rahnella aquatilis being the strain with the most traits that promote plant growth. Bioaugmentation with R. aquatilis improved seed germination, plantlet growth, and establishment of N. obliqua plantlets in the field. Specifically, bioaugmentation with R. aquatilis stimulated height (+ 52%), stem cross-sectional area (+ 89%), stomatal conductance to water vapor (+ 25%), and leaf mass area (+ 29%). These results provide evidence for the beneficial properties of seed-associated bacteria that can support the establishment of native forest tree species in the southern Andes. © 2024 Elsevier B.V., All rights reserved.
dc.description.ia_keywordgrowth, diversity, seed, establishment, associated, plant, microbial
dc.identifier.issn1573-5087
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5831
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourcePlant Growth Regulation
dc.subject.ia_odsODS 6: Agua limpia y saneamiento
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nEcología
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2024
oaire.citationEndPage336
oaire.citationIssue2
oaire.citationStartPage321
oaire.citationTitlePlant Growth Regulation
oaire.citationVolume103
oaire.fundingReferenceANID FONDEF ID23I10303
oaire.fundingReferenceUFRO DI23-0058, DI23-3009, PP23-0038
oaire.fundingReferenceANID FONDECYT 3200774
oaire.licenseConditionCopyright © Springer Nature, 2024
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication7ce95766-cf38-4e84-8ed4-e2318ac67a54
relation.isAuthorOfPublication.latestForDiscovery7ce95766-cf38-4e84-8ed4-e2318ac67a54
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionCAB Abstracts
uct.indizacionAGRICOLA
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