LH release and ovulatory response after intramuscular, intravenous, and intrauterine administration of ?-nerve growth factor of seminal plasma origin in female llamas

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datacite.alternateIdentifier.citationTheriogenology, 84 (7), 1102-1096, 2015
datacite.alternateIdentifier.doi10.1016/j.theriogenology.2015.06.006
datacite.alternateIdentifier.issn0093-691X
datacite.creatorSilva, M.
datacite.creatorFernandez, A.
datacite.creatorUlloa-Leal, C.
datacite.creatorAdams, G. P.
datacite.creatorBerland, M. A.
datacite.creatorRatto, M. H.
datacite.date2015
datacite.rightsRegistro bibliográfico
datacite.subjectllama
datacite.subjectSeminal plasma
datacite.subjectInduced ovulation
datacite.subjectbeta-Nerve growth factor
datacite.subjectCL development
datacite.titleLH release and ovulatory response after intramuscular, intravenous, and intrauterine administration of ?-nerve growth factor of seminal plasma origin in female llamas
dc.contributor.authorBERLAND OLEA, MARCO ANTONIO
dc.contributor.authorSILVA JIMENEZ, MAURICIO
dc.description.abstractThe objective of the study was to compare the pituitary and ovarian responses after intramuscular, intravenous, or intrauterine administration of P-nerve growth factor (beta-NGF) of seminal plasma origin (SP-NGF) in llamas. In experiment 1, mature female llamas with a growing follicle of 7 mm or greater were assigned randomly to four groups (n = 7/group) and given 2 mg of purified SP-NGF in a volume of 2 mL by (1) intramuscular administration,. (2) intravenous administration, and (3) intrauterine infusion, or (4) intrauterine infusion of 2 mL of PBS (negative control). Because ovulations were not detected after intrauterine infusion in experiment I, a second experiment was done to determine if a higher dose of SP-NGF given by intrauterine infusion, similar to a natural dose during copulation, will elicit an ovulatory response. In experiment 2, llamas with a growing follicle of 7 mm or greater were assigned randomly to three groups (n = 6/per group) given an intrauterine infusion of (1)4 mL of raw seminal plasma, (2)4 ml of PBS containing 20 mg of purified llama SP-NGF, or 3)4 ml of PBS (negative control). In both experiments, the ovaries were examined daily by transrectal ultrasonography using a B-mode scanner and power Doppler mode to detect ovulation and to monitor CL growth, regression, and vascularization. Blood samples were collected to determine plasma LH and progesterone concentrations. In experiment 1, only llamas treated by intramuscular or intravenous administration of SP-NGF ovulated (7 of 7 and 6 of 7, respectively). Plasma LH concentration did not differ between the intramuscular and intravenous SP-NGF-treated groups, nor did CL diameter, CL vascularization, or plasma progesterone concentration profiles. In experiment 2, the ovulation rate was 100% for llamas treated by intrauterine infusion of raw seminal plasma or llama SP-NFG, whereas no ovulations were detected in females treated with PBS. Plasma LH concentrations did not differ between groups that ovulated, nor did CL diameter, CL vascularization, or plasma progesterone concentration profiles. We conclude that beta-NGF from llama seminal plasma origin elicits a preovulatory LH surge, followed by ovulation and the development of a functional CL, regardless of the route of administration. However, the dose required to elicit pituitary and ovarian responses is higher when administered by intrauterine infusion than by intramuscular or intravenous routes. (C) 2015 Elsevier Inc. All rights reserved.
dc.description.ia_keywordplasma, intrauterine, infusion, intramuscular, intravenous, administration, llamas
dc.identifier.issn1879-3231
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/3850
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceTheriogenology
dc.subject.ia_odsODS 5: Igualdad de género
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nMatemáticas y Estadística
dc.subject.ia_oecd3nEstadística
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2015
oaire.citationEndPage1102
oaire.citationIssue7
oaire.citationStartPage1096
oaire.citationTitleTheriogenology
oaire.citationVolume84
oaire.fundingReferenceCONICYT ANID FONDECYT 1120518 (Regular)
oaire.fundingReferenceDGIP-UCT PF-2012-3-10
oaire.fundingReferenceNSERC Canada
oaire.licenseConditionCopyright © Elsevier Inc, 2015
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication3fb21919-abd0-41cd-bb97-b32be4969ad4
relation.isAuthorOfPublication5a5fa19b-bce1-4387-8139-279b35112838
relation.isAuthorOfPublication.latestForDiscovery3fb21919-abd0-41cd-bb97-b32be4969ad4
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionMEDLINE
uct.indizacionCAB Abstracts
uct.indizacionVeterinary Bulletin
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