Assemblage of Bacteria Communities and Resistome Enrichment by Dairy Flurries Along the Rhizosphere Bulk Soil Continuum on Dairy Farms

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datacite.alternateIdentifier.citationAgronomy, 15 (2), 397-397, 2025
datacite.alternateIdentifier.doi10.3390/agronomy15020397
datacite.alternateIdentifier.issn2073-4395
datacite.creatorRilling, Joaquín I.
datacite.creatorVenegas, Carmen
datacite.creatorCampos, Marco
datacite.creatorJorquera, Milko A.
datacite.creatorAcuña, Jacquelinne J.
datacite.date2025
datacite.rightsAcceso abierto
datacite.subjectResistomas ambientales
datacite.subjectPurines lácteos (o Lodos de granja lechera)
datacite.subjectResistencia a antibióticos
datacite.subjectRizosfera
datacite.subject.englishEnvironmental resistomes
datacite.subject.englishDairy slurries
datacite.subject.englishAntibiotic resistance
datacite.subject.englishRhizosphere
datacite.titleAssemblage of Bacteria Communities and Resistome Enrichment by Dairy Flurries Along the Rhizosphere Bulk Soil Continuum on Dairy Farms
dc.date.accessioned2025-08-06T18:22:38Z
dc.date.available2025-08-06T18:22:38Z
dc.description.abstractThe use of dairy slurries as organic fertilizer amendments is a common practice in agriculture as a cost-saving measure, as well as a residue management strategy. However, concerns related to the increase in antibiotic resistance in the environment under the scope of the One Health strategy are increasing. In this study, we aimed to assess resistome enrichment driven by dairy slurry application in four southern Chile dairy farms. Slurry pits, rhizospheres of Lolium perenne amended with those slurries, and bulk soils were sampled. Thirteen antibiotic-resistance genes (ARGs, tetA, tetG, tetM, tetQ, tetW, tetX, sul1, sul2, blaCTXM, blaOXA-1, blaTEM, ermB, and dfrA1) for five antibiotic classes (tetracyclines, sulfonamides, beta-lactams, macrolides, and trimethoprim sulfamethoxazole), two related integrases (intl1 and intl2), and total bacteria (16S rRNA) abundance was measured by quantitative PCR (qPCR). Then, the abundance profiles of two enzyme-inactivated ARGs (tetX and blaTEM) were determined. The differences between the bacterial communities inhabiting the different sample types were explored with 16S rRNA metabarcoding. In general, all measured ARGs were detected in slurries. A decreasing trend in ARG copy numbers was observed with increasing soil depth, with the exception of tetX, whose abundance increased in the bulk soil at specific farms. The tetX and blaTEM communities revealed no differences in the relative abundance of variants in any of the samples. Finally, taxonomic and structural differences were found among all sample types. Thus, the enrichment of the sampled farm soil resistomes was driven by the application of the raw slurries as fertilizer.
dc.description.ia_keyworddairy, were, slurries, tetx, abundance, soil, antibiotic
dc.formatPDF
dc.identifier.issn2073-4395
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6493
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute - MDPI
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAgronomy
dc.subject.ia_odsODS 2: Hambre cero
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nCiencias del Medio Ambiente
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2025
oaire.citationIssue2
oaire.citationStartPage397
oaire.citationTitleAgronomy
oaire.citationVolume15
oaire.fundingReferenceANID FONDECYT 3210594 (Postdoctorado), 1240602, 1221228 (Regular), ACT210044 (ACT)
oaire.fundingReferenceMillennium Institute Center for Genome Regulation (IM-CRG) ICN2021_044
oaire.licenseConditionObra bajo licencia Creative Commons Atribución-No Comercial-Sin Derivadas 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
uct.catalogadorjvu
uct.comunidadCiencias de la Saluden_US
uct.departamentoDepartamento de Procesos Diagnósticos y Evaluación
uct.facultadFacultad de Ciencias de la Salud
uct.indizacionSocial Sciences Citation Index - SSCI
uct.indizacionScopus
uct.indizacionLatindex
uct.indizacionDialnet
uct.indizacionScIELO
uct.indizacionDOAJ
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