Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation

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datacite.alternateIdentifier.citationAnimal Reproduction Science, 204, 59-50, 2019
datacite.alternateIdentifier.doi10.1016/j.anireprosci.2019.03.004
datacite.alternateIdentifier.issn0378-4320
datacite.creatorDiaz, Rommy
datacite.creatorLee-Estevez, Manuel
datacite.creatorQuinones, John
datacite.creatorDumorne, Kelly
datacite.creatorShort, Stefania
datacite.creatorUlloa-Rodriguez, Patricio
datacite.creatorValdebenito, Ivan
datacite.creatorSepulveda, Nestor
datacite.creatorFarias, Jorge G.
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectUltrastructure
datacite.subjectFish spermatozoa
datacite.subjectFatty acids
datacite.subjectCholesterol
datacite.titleChanges in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:39Z
dc.date.available2025-10-06T14:21:39Z
dc.description.abstractThe cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to evaluate the morphology and lipid composition of Atlantic salmon (Salmo solar) spermatozoa after storage at 4 degrees C and cryopreservation. Semen samples were obtained by stripping. One aliquot was stored at 4 degrees C for 7 days, and another aliquot was cryopreserved. The morphology and ultrastructure were analysed using electron microscopy. The lipid composition was analysed by gas chromatography and a commercial kit. After cold storage, the mitochondrion was the most affected component; however, plasma membrane rupture and detachment of the flagellum were also observed. Morphological abnormalities were greater in cryopreserved spermatozoa. The head and mid-piece were dehydrated, sperm membranes were vesiculated, and alterations of mitochondria were observed. After cold storage and cryopreservation, there were less polyunsaturated and omega-3 fatty acids. Furthermore, there was an increase in saturated fatty acids and decrease in cholesterol concentration after cryopreservation (P < 0.05). Based on the results, cryopreservation drastically damaged sperm membranes; the cryogenic damage was associated with membrane lipid composition alterations. The sperm membranes were affected less by cold storage but there was also a decrease of some lipids; therefore, there is a need for improvement in cold storage processes to decrease structural damage of spermatozoa so that semen cryopreservation can be effectively used in the salmon industry.
dc.description.ia_keywordwere, storage, cryopreservation, cold, sperm, spermatozoa, decrease
dc.identifier.issn1873-2232
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6725
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceAnimal Reproduction Science
dc.subject.ia_odsODS 7: Energía asequible y no contaminante
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationEndPage59
oaire.citationStartPage50
oaire.citationTitleAnimal Reproduction Science
oaire.citationVolume204
oaire.fundingReferenceCONICYT ANID FONDECYT 3160572 (Postdoctorado), 1180387 (Regular)
oaire.licenseConditionCopyright © Elsevier Inc, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscoverya18558bc-bf10-4e11-9254-4d4be1564d44
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus|PubMed
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