Cell Junction Pathology of Neural Stem Cells Is Associated With Ventricular Zone Disruption, Hydrocephalus, and Abnormal Neurogenesis

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datacite.alternateIdentifier.citationJournal of Neuropathology and Experimental Neurology, 74 (7), 671-653, 2015
datacite.alternateIdentifier.doi10.1097/NEN.0000000000000203
datacite.alternateIdentifier.issn0022-3069
datacite.creatorMontserrat Guerra, Maria
datacite.creatorHenzi, Roberto
datacite.creatorOrtloff, Alexander
datacite.creatorLichtin, Nicole
datacite.creatorVio, Karin
datacite.creatorJimenez, Antonio J.
datacite.creatorDolores Dominguez-Pinos, Maria
datacite.creatorGonzalez, Cesar
datacite.creatorClara Jara, Maria
datacite.creatorHinostroza, Fernando
datacite.creatorRodriguez, Sara
datacite.creatorJara, Maryoris
datacite.creatorOrtega, Eduardo
datacite.creatorGuerra, Francisco
datacite.creatorSival, Deborah A.
datacite.creatorden Dunnen, Wilfred F. A.
datacite.creatorPerez-Figares, Jose M.
datacite.creatorMcAllister, James P.
datacite.creatorJohanson, Conrad E.
datacite.creatorRodriguez, Esteban M.
datacite.date2015
datacite.rightsRegistro bibliográfico
datacite.subjectCerebrospinal fluid
datacite.subjectCongenital hydrocephalus
datacite.subjectHTx rat
datacite.subjectHuman
datacite.subjectJunction pathology
datacite.subjectNeural stem cells
datacite.subjectNeurospheres
datacite.subjectVentricular zone disruption
datacite.titleCell Junction Pathology of Neural Stem Cells Is Associated With Ventricular Zone Disruption, Hydrocephalus, and Abnormal Neurogenesis
dc.contributor.authorORTLOFF TRAUTMANN, ALEXANDER
dc.description.abstractFetal-onset hydrocephalus affects 1 to 3 per 1,000 live births. It is not only a disorder of cerebrospinal fluid dynamics but also a brain disorder that corrective surgery does not ameliorate. We hypothesized that cell junction abnormalities of neural stem cells (NSCs) lead to the inseparable phenomena of fetal-onset hydrocephalus and abnormal neurogenesis. We used bromodeoxyuridine labeling, immunocytochemistry, electron microscopy, and cell culture to study the telencephalon of hydrocephalic HTx rats and correlated our findings with those in human hydrocephalic and nonhydrocephalic human fetal brains (n = 12 each). Our results suggest that abnormal expression of the intercellular junction proteins N-cadherin and connexin-43 in NSC leads to 1) disruption of the ventricular and subventricular zones, loss of NSCs and neural progenitor cells; and 2) abnormalities in neurogenesis such as periventricular heterotopias and abnormal neuroblast migration. In HTx rats, the disrupted NSC and progenitor cells are shed into the cerebrospinal fluid and can be grown into neurospheres that display intercellular junction abnormalities similar to those of NSC of the disrupted ventricular zone; nevertheless, they maintain their potential for differentiating into neurons and glia. These NSCs can be used to investigate cellular and molecular mechanisms underlying this condition, thereby opening the avenue for stem cell therapy.
dc.description.ia_keywordcell, junction, cells, abnormal, fetal, hydrocephalus, abnormalities
dc.identifier.issn1554-6578
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4028
dc.language.isoen
dc.publisherOxford University Press
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceJournal of Neuropathology and Experimental Neurology
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2015
oaire.citationEndPage671
oaire.citationIssue7
oaire.citationStartPage653
oaire.citationTitleJournal of Neuropathology and Experimental Neurology
oaire.citationVolume74
oaire.fundingReferenceANID FONDECYT 1070241, 1111018 (Regular)
oaire.fundingReferenceHydrocephalus Association Investigator Award 51002705
oaire.fundingReferenceInstituto de Salud Carlos III PI12/0631
oaire.fundingReferenceFEDER (UE)
oaire.fundingReferenceMinisterio de Educación y Ciencia España PCI2006-A/-0669
oaire.fundingReferenceUACh DID S-2006-72
oaire.licenseConditionCopyright © the American Association of Neuropathologists, Inc, 2015
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication798755f1-1bb9-449a-b2e2-d4bbe38ca016
relation.isAuthorOfPublication.latestForDiscovery798755f1-1bb9-449a-b2e2-d4bbe38ca016
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed/MEDLINE
uct.indizacionEMBASE
uct.indizacionNeuroscience Citation Index
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