Effect of exogenous lipids on cryotolerance of Atlantic salmon (Salmo salar) spermatozoa

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datacite.alternateIdentifier.citationCryobiology, 98, 32-25, 2021
datacite.alternateIdentifier.doi10.1016/j.cryobiol.2021.01.004
datacite.alternateIdentifier.issn1090-2392
datacite.creatorDíaz, Rommy
datacite.creatorQuiñones, John A.
datacite.creatorShort, Stefania E.
datacite.creatorContreras, Pablo
datacite.creatorUlloa-Rodríguez, Patricio H.
datacite.creatorCancino-Baier, D. E.
datacite.creatorSepúlveda B., Néstor G.
datacite.creatorValdebenito, Iván
datacite.creatorFarias, Jorge G.
datacite.date2021
datacite.rightsRegistro bibliográfico
datacite.subjectArachidonic Acid
datacite.subjectAtlantic Salmon
datacite.subjectCholesterol
datacite.subjectFatty Acids
datacite.subjectFertility Rates
datacite.subjectFish Spermatozoa
datacite.subjectSperm Membrane
datacite.subjectArachidonic Acid
datacite.subjectCholesterol
datacite.subjectCyclodextrin
datacite.subjectLinoleic Acid
datacite.subjectOleic Acid
datacite.subjectSuperoxide
datacite.subjectLipid
datacite.subjectCryoprotective Agents
datacite.subjectLipids
datacite.subjectArachidonic Acid
datacite.subjectCholesterol
datacite.subjectCyclodextrin
datacite.subjectLinoleic Acid
datacite.subjectOleic Acid
datacite.subjectSuperoxide
datacite.subjectCryoprotective Agent
datacite.subjectLipid
datacite.subjectArticle
datacite.subjectCell Membrane
datacite.subjectCold Tolerance
datacite.subjectConcentration (parameter)
datacite.subjectCryopreservation
datacite.subjectFlow Cytometry
datacite.subjectMale Fertility
datacite.subjectMitochondrial Membrane Potential
datacite.subjectNonhuman
datacite.subjectPriority Journal
datacite.subjectSalmo Salar
datacite.subjectSemen Analysis
datacite.subjectSperm Function
datacite.subjectSperm Preservation
datacite.subjectSperm Quality
datacite.subjectSpermatozoon Motility
datacite.subjectThawing
datacite.subjectAnimal
datacite.subjectHuman
datacite.subjectMale
datacite.subjectProcedures
datacite.subjectSpermatozoon
datacite.subjectVeterinary Medicine
datacite.subjectAnimals
datacite.subjectCryopreservation
datacite.subjectCryoprotective Agents
datacite.subjectHumans
datacite.subjectLipids
datacite.subjectMale
datacite.subjectSemen Analysis
datacite.subjectSemen Preservation
datacite.subjectSperm Motility
datacite.subjectSpermatozoa
datacite.titleEffect of exogenous lipids on cryotolerance of Atlantic salmon (Salmo salar) spermatozoa
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.description.abstractThe development of semen cryopreservation strategies is necessary to improve the semen storage technologies of species of great commercial interest for aquaculture. Recent studies demonstrate that lipids play an important role in the fertility and cryotolerance of fish gametes. This study investigated the effect of exogenous lipids in the freezing medium on the post-thaw functional parameters of Salmo salar spermatozoa. Semen samples (n = 12) were incubated in standard extender supplemented with different concentrations of oleic acid (OA, C18:1n9), linoleic acid (LA, C18:2n6), arachidonic acid (ARA, C20:4n6) and cholesterol-loaded cyclodextrin (CLC). Post-thaw motility, membrane integrity, mitochondrial membrane potential (??m), superoxide anion (O<inf>2</inf> -) and fertility rates were analyzed. The results revealed that the semen incubated with 0.003 mmol/L OA increased the motility (~7%) and ??m (~2%) (P < 0.05), but membrane integrity and fertility were not increased. The addition of 0.003 mmol/L LA increased the motility (~4%) and all LA extenders increased the ??m (P < 0.05); however, LA increased the O<inf>2</inf> - levels and decreased the membrane integrity and fertility (P < 0.05). Semen incubated with ARA improved sperm motility (~5%), membrane integrity (~10.5%) and fertility rates (~11%) (P < 0.05). The maximum improvement in post-thaw sperm functionality was observed by adding 0.003 mmol/L ARA. In contrast, sperm quality parameters and fertility were decreased by the CLC addition (P < 0.05). This study showed that ARA could be considered as an additive for semen cryopreservation and could be relevant in the reproductive process and reproductive management of Salmo salar. © 2021 Elsevier B.V., All rights reserved.
dc.description.ia_keywordsemen, fertility, membrane, increased, were, motility, integrity
dc.identifier.issn0011-2240
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4401
dc.language.isoen
dc.publisherAcademic Press
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceCryobiology
dc.subject.ia_odsODS 14: Vida submarina
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nEcología
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2021
oaire.citationEndPage32
oaire.citationStartPage25
oaire.citationTitleCryobiology
oaire.citationVolume98
oaire.fundingReferenceANID FONDECYT 3160572, 11190621, 3190287, 1180387
oaire.licenseConditionCopyright © Elsevier Inc, 2021
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionEMBASE
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