Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa

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datacite.alternateIdentifier.citationAnimal Reproduction Science, 216, 2020
datacite.alternateIdentifier.doi10.1016/j.anireprosci.2020.106344
datacite.alternateIdentifier.issn0378-4320
datacite.creatorBravo, Williams
datacite.creatorDumorne, Kelly
datacite.creatorBeltran Lissabet, Jorge
datacite.creatorJara-Seguel, Pedro
datacite.creatorRomero, Jaime
datacite.creatorFarias, Jorge G.
datacite.creatorRisopatron, Jennie
datacite.creatorValdebenito, Ivan
datacite.creatorFigueroa, Elias
datacite.date2020
datacite.rightsRegistro bibliográfico
datacite.subjectTesticular spermatozoa
datacite.subjectPercoll gradient
datacite.subjectMotility
datacite.subjectDelta Psi Mit
datacite.subjectFertility
datacite.titleEffects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa
dc.contributor.authorFIGUEROA VILLALOBOS, ELIAS GUSTAVO
dc.contributor.authorVALDEBENITO ISLER, NEMESIO IVAN
dc.date.accessioned2025-10-06T14:21:39Z
dc.date.available2025-10-06T14:21:39Z
dc.description.abstractThe aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (Delta Psi Mit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into three groups: Control (C), T1 (45/90 % Percoll (R)) and T2 (45/60 % Percoll (R)). Sperm motility was evaluated using CASA (Computer-Assisted Sperm Analysis), Delta Psi Mit using flow cytometry, and fertility evaluating whether cleavage of fertilised eggs had occurred after 16 h of incubation at 10 degrees C. Results indicate that motility was greater in T1 (92 +/- 2.91 %) and T2 (89 +/- 2.88 %) than in the Control (83.2 +/- 2.04 %). The percentage of Delta Psi Mit was 88.3 +/- 0.58 % and 85 +/- 2% for T1 and T2, respectively, compared to 35 +/- 6.24 % for the control. The fertility rates were 76 +/- 9.1 % and 70 +/- 8.1 % for T1 and T2, respectively, compared with 66 +/- 12 % for the control. The kinetic characteristics for T1 were curvilinear velocity (VCL): 92.44 +/- 21.12 mu m/s, average path velocity (VAP): 85.87 +/- 21.83 mu m/s; and for T2 VCL was 78.69 +/- 17.63 mu m/s and VAP was 73.62 +/- 17.08 mu m/s. The results indicate sperm motility and Delta Psi Mit were greater in T1 and T2 compared with the control (P < 0.05). Similarly, there was an increase in the fertilisation rate compared to the control. The results from this study are the first where sperm quality variables were evaluated for Salmo salar testicular sperm using the Percoll (R) density gradient method.
dc.description.ia_keywordcontrol, percoll, motility, were, sperm, delta, fertility
dc.identifier.issn1873-2232
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6726
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceAnimal Reproduction Science
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nMatemáticas y Estadística
dc.subject.ia_oecd3nMatemáticas
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2020
oaire.citationTitleAnimal Reproduction Science
oaire.citationVolume216
oaire.fundingReferenceCONICYT ANID FONDECYT 3180765 (Postdoctorado), 1180387 (Regular)
oaire.fundingReferenceUCT Proyecto Interno 4136-2018
oaire.fundingReferenceUFRO Postdoctoral 2019
oaire.licenseConditionCopyright © Elsevier Inc, 2020
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication9cc0cf1e-6725-4539-8f65-58f2c6a85700
relation.isAuthorOfPublicationa18558bc-bf10-4e11-9254-4d4be1564d44
relation.isAuthorOfPublication.latestForDiscovery9cc0cf1e-6725-4539-8f65-58f2c6a85700
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus|PubMed
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