Unfurling an improved method for visualizing mitotic chromosomes in ferns

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datacite.alternateIdentifier.citationApplications in Plant Sciences, Vol.12, N°4, -, 2024.
datacite.alternateIdentifier.doi10.1002/aps3.11588
datacite.alternateIdentifier.issn2168-0450
datacite.creatorRamirez Castillo, Rosa
datacite.creatorPalma Rojas, Claudio
datacite.creatorSeguel, Pedro Jara
datacite.creatorGrusz, Amanda L.
datacite.creatorAraya Jaime, Cristian
datacite.date2024
datacite.rightsAcceso abierto
datacite.subjectCheilanthes Hypoleuca
datacite.subjectCheilanthes Mollis
datacite.subjectCheilanthoideae
datacite.subjectCitología
datacite.subjectCariotipo
datacite.subjectNephrolepidaceae
datacite.subjectNephrolepis Cordifolia
datacite.subjectDuplicación Genómica Completa
datacite.subject.englishCheilanthes hypoleuca
datacite.subject.englishCheilanthes mollis
datacite.subject.englishCheilanthoideae
datacite.subject.englishcytology
datacite.subject.englishkaryotype
datacite.subject.englishNephrolepidaceae
datacite.subject.englishNephrolepis cordifolia
datacite.subject.englishwhole-genome duplication
datacite.titleUnfurling an improved method for visualizing mitotic chromosomes in ferns
dc.date.accessioned9/10/24 15:47
dc.date.available9/10/24 15:47
dc.description.abstractPremise: Cytotaxonomy employs chromosome visualization to study organismal relationships and evolution. Despite the critical value of cytogenetic data, cytotypes are lacking for many plant groups. Here, we present an improved approach for visualizing mitotic chromosomes in ferns, a key lineage of land plants, using the dividing cells of unfurling croziers (fiddleheads). Methods and Results: Our modified mitotic chromosome preparation incorporates a brief pectinase-cellulase pretreatment, as well as colchicine fixation and the Feulgen reaction to improve the staining and separation of mitotic chromosomes. To demonstrate this easy and efficient assessment, we determined the sporophytic (2n) chromosome number for three fern species: Cheilanthes mollis (2n = 60), Cheilanthes hypoleuca (2n = 120), and Nephrolepis cordifolia (2n = 82). Conclusions: The new method presented here improves visualizations of mitotic chromosomes from the dividing nuclei of young fern croziers. Fiddleheads are widely accessible in nature and in living collections worldwide, and this modified approach increases their suitability for fern cytotaxonomic studies.
dc.description.ia_keywordmitotic, chromosomes, chromosome, fern, here, improved, approach
dc.formatPDF
dc.identifier.issn2168-0450
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5998
dc.language.isoen
dc.publisherWiley
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceApplications in Plant Sciences
dc.subject.ia_odsODS 15: Vida de ecosistemas terrestres
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2024
oaire.citationIssue4
oaire.citationTitleApplications in Plant Sciences
oaire.citationVolume12
oaire.fundingReferenceANID 21220782 (Beca Doctorado)
oaire.fundingReferenceNSF DEB2232106
oaire.fundingReferenceInstituto de Ecología y Biodiversidad (IEB) FB210006 (BASAL)
oaire.licenseConditionObra bajo licencia Creative Commons Atribución-No Comercial 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by-nc/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Biológicas y Químicas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionSCOPUS
uct.indizacionDOAJ
uct.indizacionWOS
uct.indizacionPubMed
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